ZNF580 CRISPR/Cas9 KO Plasmid (h): sc-406194
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZNF580 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZNF580 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF580 CRISPR/Cas9 KO Plasmid (h) | sc-406194 | 20 µg | $397.00 |
Overview
ZNF580 is a KRAB-containing C2H2 zinc finger protein that functions primarily as a transcriptional regulator, linking sequence-specific DNA binding to chromatin-modifying complexes that shape gene expression programs. It has been implicated in controlling endothelial cell behavior, including migration and angiogenic responses, and in coordinating oxidative stress and inflammatory signaling outputs. Through these regulatory roles, ZNF580 can influence pathways governing vascular remodeling, cell-cycle progression, and stress-responsive transcription. Dysregulated ZNF580 expression or activity has been associated with vascular pathology contexts such as atherosclerosis-related processes and ischemia-linked endothelial dysfunction, making it a useful node for mechanistic studies of vascular biology.
ZNF580 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZNF580 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZNF580 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZNF580 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZNF580 protein expression.
This CRISPR knockout system enables efficient generation of ZNF580-deficient cell models for investigation of ZNF580 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZNF580 exon(s) critical for ZNF580 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZNF580 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZNF580 CRISPR/Cas9 KO Plasmid (h) and ZNF580 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZNF580 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZNF580 HDR Plasmid (h) and ZNF580 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZNF580 homology arms to support homology-directed repair at defined ZNF580 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.