ZNF35 Double Nickase Plasmid (h): sc-411079-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZNF35 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZNF35 Double Nickase Plasmid (h) and ZNF35 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZNF35. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ZNF35 Antibody (C-11): sc-376972
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF35 Double Nickase Plasmid (h) | sc-411079-NIC | 20 µg | $410.00 | |||
ZNF35 Double Nickase Plasmid (h2) | sc-411079-NIC-2 | 20 µg | $410.00 |
ZNF35 encodes a KRAB-domain C2H2 zinc finger protein implicated in sequence-specific DNA binding and transcriptional repression through recruitment of co-repressor complexes such as KAP1/TRIM28, promoting heterochromatin formation and epigenetic silencing. As part of broader zinc finger–mediated gene regulatory networks, ZNF35 is positioned to influence chromatin organization, RNA polymerase II transcriptional control, and cell state maintenance programs. Altered expression or regulatory variation in KRAB-ZNF factors has been associated with dysregulated transcriptional programs observed in cancer and other diseases, making ZNF35 a useful node for studying transcriptional circuitry and epigenetic control in human cells. Functional interrogation of ZNF35 can help clarify how KRAB-ZNF proteins contribute to lineage-specific gene repression and stability of gene expression patterns.
ZNF35 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZNF35 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZNF35. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZNF35 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZNF35-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.