ZNF174 Antibody (C-10): sc-398744
- ZNF174 Antibody (C-10) is a mouse monoclonal IgG1 κ ZNF174 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 233-259 within an internal region of ZNF174 of mouse origin
- ZNF174 Antibody (C-10) is recommended for detection of ZNF174 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ZNF174 Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ZNF174 Antibody (C-10) for WB applications. These reagents are now offered in bundles with ZNF174 Antibody (C-10) (see ordering information below).
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ZNF174 Antibody (C-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects ZNF174 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZNF174 Antibody (C-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZNF174, also known as ZSCAN8 or AW-1, functions as a transcriptional repressor and is a member of the Krüppel C2H2-type zinc-finger protein family. ZNF174 plays a crucial role in regulating gene expression by repressing promoter activities of key growth factors such as PDGF-B and TGFβ1, which are involved in cellular proliferation and differentiation. ZNF174 is predominantly expressed in adult ovary and testis, but can also be found in various tissues including small intestine, prostate, colon, spleen, pancreas, skeletal muscle, brain, heart, kidney, and thymus. ZNF174 is localized in the nucleus and features three C2H2-type zinc fingers at C-terminus and one SCAN domain near N-terminus. The SCAN domain facilitates formation of noncovalent complexes among zinc finger proteins, potentially influencing oligomerization and functional interactions, making ZNF174 a vital component in regulation of transcriptional networks and underscoring its importance in developmental and physiological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ZNF174 Antibody (C-10) References:
- The zinc finger-associated SCAN box is a conserved oligomerization domain. | Williams, AJ., et al. 1999. Mol Cell Biol. 19: 8526-35. PMID: 10567577
- The SCAN domain mediates selective oligomerization. | Schumacher, C., et al. 2000. J Biol Chem. 275: 17173-9. PMID: 10747874
- The SCAN domain of ZNF174 is a dimer. | Stone, JR., et al. 2002. J Biol Chem. 277: 5448-52. PMID: 11741982
- The SCAN domain defines a large family of zinc finger transcription factors. | Sander, TL., et al. 2003. Gene. 310: 29-38. PMID: 12801630
- Mammalian SCAN domain dimer is a domain-swapped homolog of the HIV capsid C-terminal domain. | Ivanov, D., et al. 2005. Mol Cell. 17: 137-43. PMID: 15629724
- The SCAN domain family of zinc finger transcription factors. | Edelstein, LC. and Collins, T. 2005. Gene. 359: 1-17. PMID: 16139965
- The structurally disordered KRAB repression domain is incorporated into a protease resistant core upon binding to KAP-1-RBCC domain. | Peng, H., et al. 2007. J Mol Biol. 370: 269-89. PMID: 17512541
- Repression of vascular endothelial growth factor expression by the zinc finger transcription factor ZNF24. | Harper, J., et al. 2007. Cancer Res. 67: 8736-41. PMID: 17875714
- Isolation and characterization of a novel zinc-finger protein with transcription repressor activity. | Williams, AJ., et al. 1995. J Biol Chem. 270: 22143-52. PMID: 7673192
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF174 Antibody (C-10) | sc-398744 | 200 µg/ml | $316.00 | |||
ZNF174 Antibody (C-10): m-IgG Fc BP-HRP Bundle | sc-527807 | 200 µg Ab; 10 µg BP | $354.00 | |||
ZNF174 Antibody (C-10): m-IgG1 BP-HRP Bundle | sc-533180 | 200 µg Ab; 20 µg BP | $354.00 | |||
ZNF174 Antibody (C-10) AC | sc-398744 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ZNF174 Antibody (C-10) HRP | sc-398744 HRP | 200 µg/ml | $316.00 | |||
ZNF174 Antibody (C-10) FITC | sc-398744 FITC | 200 µg/ml | $330.00 | |||
ZNF174 Antibody (C-10) PE | sc-398744 PE | 200 µg/ml | $343.00 | |||
ZNF174 Antibody (C-10) Alexa Fluor® 488 | sc-398744 AF488 | 200 µg/ml | $357.00 | |||
ZNF174 Antibody (C-10) Alexa Fluor® 546 | sc-398744 AF546 | 200 µg/ml | $357.00 | |||
ZNF174 Antibody (C-10) Alexa Fluor® 594 | sc-398744 AF594 | 200 µg/ml | $357.00 | |||
ZNF174 Antibody (C-10) Alexa Fluor® 647 | sc-398744 AF647 | 200 µg/ml | $357.00 | |||
ZNF174 Antibody (C-10) Alexa Fluor® 680 | sc-398744 AF680 | 200 µg/ml | $357.00 | |||
ZNF174 Antibody (C-10) Alexa Fluor® 790 | sc-398744 AF790 | 200 µg/ml | $357.00 | |||
ZNF174 (C-10) Neutralizing Peptide | sc-398744 P | 100 µg/0.5 ml | $68.00 |