ZNF136 Double Nickase Plasmid (h): sc-411093-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZNF136 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZNF136 Double Nickase Plasmid (h) and ZNF136 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZNF136. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ZNF136 Antibody (ZNF1H5D11): sc-81133
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF136 Double Nickase Plasmid (h) | sc-411093-NIC | 20 µg | $410.00 | |||
ZNF136 Double Nickase Plasmid (h2) | sc-411093-NIC-2 | 20 µg | $410.00 |
ZNF136 encodes a KRAB domain–containing C2H2 zinc-finger protein implicated in sequence-specific DNA binding and transcriptional repression through recruitment of corepressor complexes. As part of zinc-finger regulatory networks, ZNF136 is expected to influence chromatin state and gene expression programs that coordinate cell differentiation, proliferation, and stress-responsive transcription. Dysregulation of KRAB-ZNF–mediated silencing has been associated with altered epigenetic control and transcriptional noise in diverse disease contexts, making ZNF136 of interest for studies of gene regulatory stability. Functional interrogation of ZNF136 supports mechanistic work on transcriptional control, chromatin-associated pathways, and downstream effects on cellular phenotypes relevant to biomedical research.
ZNF136 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZNF136 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZNF136. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZNF136 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZNF136-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.