ZNF131 CRISPR/Cas9 KO Plasmid (h): sc-406166
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZNF131 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZNF131 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF131 CRISPR/Cas9 KO Plasmid (h) | sc-406166 | 20 µg | $397.00 |
Overview
ZNF131 encodes a KRAB-domain zinc finger transcription factor implicated in sequence-specific DNA binding and chromatin-associated gene repression through recruitment of corepressor complexes. By modulating transcriptional programs linked to cell-cycle control, differentiation, and stress responses, ZNF131 can influence pathways that shape nuclear organization and epigenetic stability. Altered regulation of KRAB-ZNF networks has been associated with dysregulated transcriptional states observed across multiple disease contexts, including cancer and neurodevelopmental phenotypes. As a nuclear regulatory protein, ZNF131 is frequently studied for its role in transcriptional control, chromatin dynamics, and context-dependent effects on cell identity.
ZNF131 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZNF131 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZNF131 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZNF131 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZNF131 protein expression.
This CRISPR knockout system enables efficient generation of ZNF131-deficient cell models for investigation of ZNF131 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZNF131 exon(s) critical for ZNF131 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZNF131 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZNF131 CRISPR/Cas9 KO Plasmid (h) and ZNF131 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZNF131 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZNF131 HDR Plasmid (h) and ZNF131 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZNF131 homology arms to support homology-directed repair at defined ZNF131 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.