Zic2 CRISPR/Cas9 KO Plasmid (h): sc-404720
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Zic2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Zic2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Zic2 Antibody (3C12): sc-517055
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Zic2 CRISPR/Cas9 KO Plasmid (h) | sc-404720 | 20 µg | $397.00 |
Overview
ZIC2 encodes the zinc finger transcription factor Zic2, a key regulator of embryonic patterning and neural development that modulates gene expression programs controlling cell fate specification, proliferation, and differentiation. Zic2 functions within developmental transcriptional networks and intersects with signaling pathways that shape morphogenesis, including pathways influencing neural tube formation and forebrain midline patterning. Altered ZIC2 activity is linked to congenital neurodevelopmental disorders, most notably holoprosencephaly, and dysregulated expression has been reported in studies of tumor biology and lineage plasticity. As a DNA-binding transcriptional regulator, Zic2 provides a mechanistic entry point for investigating enhancer–promoter control, chromatin-dependent transcription, and developmental gene regulatory circuits in human cells.
Zic2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZIC2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZIC2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZIC2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Zic2 protein expression.
This CRISPR knockout system enables efficient generation of ZIC2-deficient cell models for investigation of Zic2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZIC2 exon(s) critical for Zic2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZIC2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Zic2 CRISPR/Cas9 KO Plasmid (h) and Zic2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZIC2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Zic2 HDR Plasmid (h) and Zic2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZIC2 homology arms to support homology-directed repair at defined ZIC2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.