ZHX3 Antibody (D-10): sc-514773

ZHX3 Antibody (D-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects ZHX3 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZHX3 (D-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZHX3 protein plays a crucial role as a transcriptional repressor, primarily localized in the nucleus, where ZHX3 regulates gene expression by interacting with the activation domain of the A subunit of nuclear factor-Y (NF-YA). This interaction is vital for maintaining proper cellular function and development, as dysregulation of ZHX3 can lead to various diseases, including hepatocellular carcinoma, where hypermethylation-mediated silencing of ZHX2 has been implicated. ZHX proteins, including ZHX3, are characterized by their unique structure, which consists of two Cys(2)-His(2)-type zinc-finger motifs and five homeodomains, allowing ZHX3 to form both homodimers and heterodimers. This structural versatility is essential for their function as transcriptional repressors, enabling ZHX3 to modulate a wide range of target genes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ZHX3 Antibody (D-10) References:

1. Human ZHX1: cloning, chromosomal location, and interaction with transcription factor NF-Y.  |  Yamada, K., et al. 1999. Biochem Biophys Res Commun. 261: 614-21. PMID: 10441475
2. Rat zinc-fingers and homeoboxes 1 (ZHX1), a nuclear factor-YA-interacting nuclear protein, forms a homodimer.  |  Hirano, S., et al. 2002. Gene. 290: 107-14. PMID: 12062805
3. Functional analysis and the molecular dissection of zinc-fingers and homeoboxes 1 (ZHX1).  |  Yamada, K., et al. 2002. Biochem Biophys Res Commun. 297: 368-74. PMID: 12237128
4. Genomic structure and analysis of transcriptional regulation of the mouse zinc-fingers and homeoboxes 1 (ZHX1) gene.  |  Shou, Z., et al. 2003. Gene. 302: 83-94. PMID: 12527199
5. Analysis of zinc-fingers and homeoboxes (ZHX)-1-interacting proteins: molecular cloning and characterization of a member of the ZHX family, ZHX3.  |  Yamada, K., et al. 2003. Biochem J. 373: 167-78. PMID: 12659632
6. Zinc-fingers and homeoboxes (ZHX) 2, a novel member of the ZHX family, functions as a transcriptional repressor.  |  Kawata, H., et al. 2003. Biochem J. 373: 747-57. PMID: 12741956
7. The mouse zinc-fingers and homeoboxes (ZHX) family; ZHX2 forms a heterodimer with ZHX3.  |  Kawata, H., et al. 2003. Gene. 323: 133-40. PMID: 14659886
8. Promoter hypermethylation of a novel gene, ZHX2, in hepatocellular carcinoma.  |  Lv, Z., et al. 2006. Am J Clin Pathol. 125: 740-6. PMID: 16707376

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ZHX3 Antibody (D-10) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-ZHX3 Antibody (D-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ZHX3 Antibody (D-10) Specifications

Alternate Product Name: ZHX3

Product Clone Name: D-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 389-517

Epitope Target: ZHX3

Epitope Species: human

Product Reactivity/Detection: ZHX3

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for ZHX3 Antibody (D-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin