ZFP64 Double Nickase Plasmid (h): sc-410027-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZFP64 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZFP64 Double Nickase Plasmid (h) and ZFP64 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZFP64. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ZFP64 Antibody (F-3): sc-374263
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZFP64 Double Nickase Plasmid (h) | sc-410027-NIC | 20 µg | $410.00 | |||
ZFP64 Double Nickase Plasmid (h2) | sc-410027-NIC-2 | 20 µg | $410.00 |
ZFP64 (zinc finger protein 64) is a putative KRAB-domain C2H2 zinc-finger transcription factor implicated in sequence-specific DNA binding and epigenetic regulation of gene expression. By recruiting corepressor complexes and chromatin-modifying enzymes, ZFP64 is thought to influence transcriptional silencing, chromatin state maintenance, and cell identity programs. Altered regulation of KRAB zinc-finger networks has been linked to dysregulated differentiation, genome stability, and oncogenic transcriptional states, making ZFP64 relevant to studies of cancer-associated gene regulation and developmental control. In human cells, interrogating ZFP64 function supports mechanistic analysis of transcriptional circuitry and chromatin-dependent pathways.
ZFP64 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZFP64 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZFP64. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZFP64 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZFP64-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.