SIP1/ZEB2 Antibody (E-11): sc-271984

ZEB2 Antibody (E-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects ZEB2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-ZEB2 antibody (E-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZEB2, also known as zinc finger E-box-binding protein 2, plays a pivotal role in regulating gene expression through interactions with transcription factors, particularly in epithelial-mesenchymal transition (EMT), which is crucial for processes such as embryonic development and tissue repair. ZEB2 is characterized by a unique structure, including a SMAD-binding domain, a homeodomain, and two clusters of zinc fingers at both N- and C-termini, allowing binding to specific DNA sequences and interaction with other proteins. This structural complexity is essential for mediating the effects of transforming growth factor-beta (TGF-β) signaling, vital for normal neural development and formation of neural crest cells. Mutations in the ZEB2 gene, located on chromosome 2q22, are associated with Hirschsprung disease, leading to severe developmental disorders such as mental retardation, delayed motor skills, and congenital heart defects, underscoring ZEB2′s significance in both health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SIP1/ZEB2 Antibody (E-11) References:

1. SIP1 (Smad interacting protein 1) and deltaEF1 (delta-crystallin enhancer binding factor) are structurally similar transcriptional repressors.  |  van Grunsven, LA., et al. 2001. J Bone Joint Surg Am. 83-A Suppl 1: S40-7. PMID: 11263664
2. Loss-of-function mutations in SIP1 Smad interacting protein 1 result in a syndromic Hirschsprung disease.  |  Cacheux, V., et al. 2001. Hum Mol Genet. 10: 1503-10. PMID: 11448942
3. Smad-interacting protein 1 is a repressor of liver/bone/kidney alkaline phosphatase transcription in bone morphogenetic protein-induced osteogenic differentiation of C2C12 cells.  |  Tylzanowski, P., et al. 2001. J Biol Chem. 276: 40001-7. PMID: 11477103
4. Nonsense and frameshift mutations in ZFHX1B, encoding Smad-interacting protein 1, cause a complex developmental disorder with a great variety of clinical features.  |  Yamada, K., et al. 2001. Am J Hum Genet. 69: 1178-85. PMID: 11592033
5. Large-scale deletions and SMADIP1 truncating mutations in syndromic Hirschsprung disease with involvement of midline structures.  |  Amiel, J., et al. 2001. Am J Hum Genet. 69: 1370-7. PMID: 11595972
6. The Transcription Factor ZEB2 Is Required to Maintain the Tissue-Specific Identities of Macrophages.  |  Scott, CL., et al. 2018. Immunity. 49: 312-325.e5. PMID: 30076102
7. Endothelial Zeb2 preserves the hepatic angioarchitecture and protects against liver fibrosis.  |  de Haan, W., et al. 2022. Cardiovasc Res. 118: 1262-1275. PMID: 33909875
8. Ablation of cDC2 development by triple mutations within the Zeb2 enhancer.  |  Liu, TT., et al. 2022. Nature. 607: 142-148. PMID: 35732734
9. Endothelial HDAC1-ZEB2-NuRD Complex Drives Aortic Aneurysm and Dissection Through Regulation of Protein S-Sulfhydration.  |  Luo, S., et al. 2023. Circulation. 147: 1382-1403. PMID: 36951067
10. A positive feedback loop between ZEB2 and ACSL4 regulates lipid metabolism to promote breast cancer metastasis.  |  Lin, J., et al. 2023. Elife. 12: PMID: 38078907
11. The transcription factor ZEB2 drives the formation of age-associated B cells.  |  Dai, D., et al. 2024. Science. 383: 413-421. PMID: 38271512
12. Age-associated CD4+ T cells with B cell-promoting functions are regulated by ZEB2 in autoimmunity.  |  Goto, M., et al. 2024. Sci Immunol. 9: eadk1643. PMID: 38330141

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SIP1/ZEB2 Antibody (E-11) has 80 citations in a variety of scientific publications.

Primary antibodies like Anti-SIP1/ZEB2 Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SIP1/ZEB2 Antibody (E-11) Specifications

Alternate Product Name: ZEB2

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 401-660

Epitope Target: ZEB2

Epitope Species: human

Product Reactivity/Detection: ZEB2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 80 citations in various scientific publications

Available Conjugates/Forms for SIP1/ZEB2 Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin