ZEB1 Antibody (416A7H10): sc-81428

ZEB1 Antibody (416A7H10) is a mouse monoclonal IgG1 antibody specifically engineered to detect human ZEB1 with exceptional accuracy in western blotting (WB) and immunoprecipitation (IP) applications. ZEB1 monoclonal antibody (416A7H10) is raised against a recombinant protein corresponding to a region near the N-terminus of human ZEB1, ensuring precise targeting and strong reactivity in assays involving human samples. ZEB1, also known as AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP, and ZFHX1A, is a critical non-receptor transcription factor that plays a vital role in regulating gene expression during embryonic development and tissue maturation, particularly within mesodermal tissues. ZEB1 contains two zinc finger domains essential for DNA binding and transcriptional repression, as well as a homeodomain that facilitates protein-protein interactions. ZEB1′s three repression domains enable modulation of gene expression through site competition, enhancer silencing, and interaction with corepressors, making ZEB1 a key player in processes such as histogenesis and skeletal structure development. ZEB1′s interaction with the TSHβ gene T3-response element highlights ZEB1′s importance in regulating gene-specific responses to thyroid hormones, which are crucial for metabolic and developmental functions. Through influence on these fundamental biological processes, ZEB1 is implicated in various physiological and pathological conditions, including cancer progression and cellular differentiation. Anti-ZEB1 antibody (416A7H10) is available in a non-conjugated form, providing researchers flexibility to utilize across a range of experimental protocols to advance studies on this pivotal transcription factor.

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ZEB1 Antibody (416A7H10) References:

1. Differential expression and function of members of the zfh-1 family of zinc finger/homeodomain repressors.  |  Postigo, AA. and Dean, DC. 2000. Proc Natl Acad Sci U S A. 97: 6391-6. PMID: 10841546
2. T3-activation of the rat growth hormone gene is inhibited by a zinc finger/homeodomain protein.  |  Cabanillas, AM., et al. 2001. Mol Cell Endocrinol. 181: 131-7. PMID: 11476947
3. Delta-crystallin enhancer binding protein delta EF1 is a zinc finger-homeodomain protein implicated in postgastrulation embryogenesis.  |  Funahashi, J., et al. 1993. Development. 119: 433-46. PMID: 7904558
4. BZP, a novel serum-responsive zinc finger protein that inhibits gene transcription.  |  Franklin, AJ., et al. 1994. Mol Cell Biol. 14: 6773-88. PMID: 7935395
5. DeltaEF1, a zinc finger and homeodomain transcription factor, is required for skeleton patterning in multiple lineages.  |  Takagi, T., et al. 1998. Development. 125: 21-31. PMID: 9389660
6. Two mechanisms in the action of repressor deltaEF1: binding site competition with an activator and active repression.  |  Sekido, R., et al. 1997. Genes Cells. 2: 771-83. PMID: 9544704
7. A zinc finger homeodomain transcription factor binds specific thyroid hormone response elements.  |  Darling, DS., et al. 1998. Mol Cell Endocrinol. 139: 25-35. PMID: 9705071

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ZEB1 Antibody (416A7H10) has 38 citations in a variety of scientific publications.

Primary antibodies like Anti-ZEB1 Antibody (416A7H10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ZEB1 Antibody (416A7H10) Specifications

Alternate Product Name: ZEB1

Product Clone Name: 416A7H10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: ZEB1

Epitope Species: human

Product Reactivity/Detection: ZEB1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 1.0% stabilizer protein

Research Citations: 38 citations in various scientific publications

Available Conjugates/Forms for ZEB1 Antibody (416A7H10): N/A

The preferred ZEB1 antibody is ZEB1 Antibody (H-3): sc-515797.