ZDHHC7 CRISPR/Cas9 KO Plasmid (h): sc-405072
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZDHHC7 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZDHHC7 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZDHHC7 CRISPR/Cas9 KO Plasmid (h) | sc-405072 | 20 µg | $397.00 |
Overview
ZDHHC7 encodes a DHHC family palmitoyltransferase that catalyzes S-palmitoylation of substrate proteins, a reversible lipid modification that regulates membrane association, trafficking, stability, and signaling output. By controlling palmitoylation-dependent localization of signaling and adaptor proteins, ZDHHC7 influences processes such as receptor-proximal signaling, vesicular transport, and organization of membrane microdomains. Dysregulated palmitoylation has been linked to altered growth and survival signaling, immune cell function, and neuronal physiology, making ZDHHC7 a useful node for studying post-translational lipidation networks in disease-relevant contexts. Investigating ZDHHC7 helps define how palmitoylation dynamics shape pathway crosstalk and proteostasis under stress or transformation-associated conditions.
ZDHHC7 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZDHHC7 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZDHHC7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZDHHC7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZDHHC7 protein expression.
This CRISPR knockout system enables efficient generation of ZDHHC7-deficient cell models for investigation of ZDHHC7 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZDHHC7 exon(s) critical for ZDHHC7 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZDHHC7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZDHHC7 CRISPR/Cas9 KO Plasmid (h) and ZDHHC7 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZDHHC7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZDHHC7 HDR Plasmid (h) and ZDHHC7 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZDHHC7 homology arms to support homology-directed repair at defined ZDHHC7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.