ZDHHC20 Double Nickase Plasmid (h): sc-406353-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZDHHC20 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZDHHC20 Double Nickase Plasmid (h) and ZDHHC20 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZDHHC20. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ZDHHC20 Antibody (A-4): sc-518217
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZDHHC20 Double Nickase Plasmid (h) | sc-406353-NIC | 20 µg | $410.00 | |||
ZDHHC20 Double Nickase Plasmid (h2) | sc-406353-NIC-2 | 20 µg | $410.00 |
ZDHHC20 encodes a DHHC family palmitoyltransferase that catalyzes S-palmitoylation of protein substrates, regulating membrane association, subcellular trafficking, and stability of signaling proteins. Through dynamic control of lipid modification, ZDHHC20 influences processes such as receptor signaling, vesicular transport, and organization of membrane microdomains that shape downstream pathways. Altered palmitoylation programs involving ZDHHC20 have been associated with dysregulated cell growth and migration phenotypes and have been explored in the context of cancer biology and other disorders where membrane signaling fidelity is perturbed. As a result, ZDHHC20 is a useful target for studying how palmitoylation-dependent protein localization impacts signal transduction and cellular homeostasis in human models.
ZDHHC20 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZDHHC20 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZDHHC20. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZDHHC20 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZDHHC20-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.