ZCCHC3 CRISPR/Cas9 KO Plasmid (m): sc-426824
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZCCHC3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZCCHC3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZCCHC3 CRISPR/Cas9 KO Plasmid (m) | sc-426824 | 20 µg | $397.00 |
Overview
Zcchc3 encodes ZCCHC3, a CCCH-type zinc finger protein implicated in cytosolic nucleic acid sensing and regulation of innate immune signaling. Reported functions connect ZCCHC3 to antiviral defense pathways, including modulation of RIG-I/MDA5- and cGAS–STING-associated responses that shape type I interferon and inflammatory gene programs. By influencing RNA/DNA-triggered signaling and downstream transcriptional outputs, ZCCHC3 can affect cellular responses to infection and immune stress. Dysregulation of these processes is relevant to studies of inflammatory phenotypes and host–pathogen interactions in mouse models.
ZCCHC3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Zcchc3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Zcchc3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Zcchc3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZCCHC3 protein expression.
This CRISPR knockout system enables efficient generation of Zcchc3-deficient cell models for investigation of ZCCHC3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Zcchc3 exon(s) critical for ZCCHC3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Zcchc3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZCCHC3 CRISPR/Cas9 KO Plasmid (m) and ZCCHC3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Zcchc3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZCCHC3 HDR Plasmid (m) and ZCCHC3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Zcchc3 homology arms to support homology-directed repair at defined Zcchc3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.