ZCCHC14 CRISPR/Cas9 KO Plasmid (h): sc-411686
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZCCHC14 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZCCHC14 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZCCHC14 CRISPR/Cas9 KO Plasmid (h) | sc-411686 | 20 µg | $397.00 |
Overview
ZCCHC14 encodes a nuclear zinc finger CCHC-type protein implicated in RNA-binding functions that influence post-transcriptional gene regulation, including RNA stability and processing. It has been linked to pathways governing RNA metabolism and the control of gene expression programs that shape cellular homeostasis. Emerging evidence connects ZCCHC14 to virus–host interactions, consistent with roles for zinc-binding RNA-associated factors in regulating viral RNA utilization and host transcriptome responses. Dysregulation of RNA-binding proteins and RNA processing networks is broadly relevant to oncogenic signaling and cell state transitions, making ZCCHC14 a useful node for mechanistic studies in these contexts.
ZCCHC14 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZCCHC14 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZCCHC14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZCCHC14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZCCHC14 protein expression.
This CRISPR knockout system enables efficient generation of ZCCHC14-deficient cell models for investigation of ZCCHC14 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZCCHC14 exon(s) critical for ZCCHC14 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZCCHC14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZCCHC14 CRISPR/Cas9 KO Plasmid (h) and ZCCHC14 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZCCHC14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZCCHC14 HDR Plasmid (h) and ZCCHC14 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZCCHC14 homology arms to support homology-directed repair at defined ZCCHC14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.