ZCCHC11 CRISPR/Cas9 KO Plasmid (h): sc-411714
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZCCHC11 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZCCHC11 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZCCHC11 CRISPR/Cas9 KO Plasmid (h) | sc-411714 | 20 µg | $397.00 |
Overview
ZCCHC11 (also known as TUT4) encodes a zinc finger CCHC-type RNA uridyltransferase that catalyzes 3′ terminal uridylation of diverse RNA substrates, including precursor and mature microRNAs and select mRNAs. By modifying RNA 3′ ends, ZCCHC11 influences RNA stability and decay pathways, miRNA biogenesis, and post-transcriptional gene regulation, with functional interplay involving LIN28-dependent control of let-7 family miRNAs. This activity connects ZCCHC11 to regulatory programs governing differentiation, proliferation, and stress-responsive transcriptome remodeling. Dysregulated ZCCHC11 expression or activity has been studied in contexts of aberrant miRNA networks and oncogenic signaling, making it relevant for mechanistic studies in cancer biology and RNA metabolism.
ZCCHC11 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZCCHC11 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZCCHC11 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZCCHC11 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZCCHC11 protein expression.
This CRISPR knockout system enables efficient generation of ZCCHC11-deficient cell models for investigation of ZCCHC11 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZCCHC11 exon(s) critical for ZCCHC11 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZCCHC11 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZCCHC11 CRISPR/Cas9 KO Plasmid (h) and ZCCHC11 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZCCHC11 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZCCHC11 HDR Plasmid (h) and ZCCHC11 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZCCHC11 homology arms to support homology-directed repair at defined ZCCHC11 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.