ZBTB5 CRISPR/Cas9 KO Plasmid (m): sc-432923
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZBTB5 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZBTB5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZBTB5 CRISPR/Cas9 KO Plasmid (m) | sc-432923 | 20 µg | $397.00 |
Overview
Zbtb5 encodes ZBTB5, a BTB/POZ and C2H2 zinc-finger transcription factor that supports sequence-specific DNA binding and assembly of regulatory complexes controlling gene expression programs. In mouse cells, ZBTB family factors are commonly linked to chromatin-dependent transcriptional repression or context-dependent activation through recruitment of coregulators, shaping processes such as cell-cycle progression, lineage commitment, and cellular differentiation. By influencing transcriptional networks that govern proliferation and developmental state, ZBTB5 is relevant to mechanistic studies of dysregulated gene regulation observed in oncogenic transformation and other transcription-associated pathologies. Functional interrogation of Zbtb5 can clarify how BTB–zinc finger proteins integrate promoter and enhancer activity to coordinate downstream pathways.
ZBTB5 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Zbtb5 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Zbtb5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Zbtb5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZBTB5 protein expression.
This CRISPR knockout system enables efficient generation of Zbtb5-deficient cell models for investigation of ZBTB5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Zbtb5 exon(s) critical for ZBTB5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Zbtb5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZBTB5 CRISPR/Cas9 KO Plasmid (m) and ZBTB5 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Zbtb5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZBTB5 HDR Plasmid (m) and ZBTB5 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Zbtb5 homology arms to support homology-directed repair at defined Zbtb5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.