Date published: 2025-10-17

1-800-457-3801

SCBT Portrait Logo
Seach Input

ZBTB20 Antibody (E-11): sc-515370

5.0(2)
Write a reviewAsk a question

Datasheets
  • ZBTB20 Antibody (E-11) is a mouse monoclonal IgG1 ZBTB20 antibody, cited in 2 publications, provided at 200 µg/ml
  • raised against amino acids 383-605 mapping within an internal region of ZBTB20 of human origin
  • ZBTB20 Antibody (E-11) is recommended for detection of ZBTB20 of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-ZBTB20 Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • TransCruz reagent for ChIP application (sc-515370 X, 200 µg/0.1 ml)
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ZBTB20 Antibody (E-11) for WB applications. These reagents are now offered in bundles with ZBTB20 Antibody (E-11) (see ordering information below).

QUICK LINKS

SEE ALSO...

ZBTB20 Antibody (E-11) is a mouse monoclonal IgG1 antibody that detects ZBTB20 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZBTB20 (E-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZBTB20, also known as zinc finger and BTB domain containing 20, is a 741 amino acid transcription factor that plays a crucial role in regulating gene expression, particularly in hematopoiesis and immune responses. ZBTB20 is characterized by nuclear localization and contains one BTB (POZ) domain along with five C2H2-type zinc fingers, which are essential for DNA-binding capabilities. ZBTB20′s ability to exist as either a monomer or a homodimer enhances functional versatility, allowing modulation of transcriptional activity in various biological contexts. Additionally, multiple isoforms resulting from alternative splicing events further underscore the complexity of regulatory functions. Anti-ZBTB20 antibody (E-11) helps researchers gain valuable insights into mechanisms of oncogenesis and immune system regulation, making this tool indispensable for studies in molecular biology and immunology.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

ZBTB20 Antibody (E-11) References:

  1. Assignment of the human zinc finger gene, ZNF288, to chromosome 3 band q13.2 by radiation hybrid mapping and fluorescence in situ hybridisation.  |  Harboe, TL., et al. 2000. Cytogenet Cell Genet. 89: 156-7. PMID: 10965110
  2. Identification and characterization of DPZF, a novel human BTB/POZ zinc finger protein sharing homology to BCL-6.  |  Zhang, W., et al. 2001. Biochem Biophys Res Commun. 282: 1067-73. PMID: 11352661
  3. Characterization of two novel nuclear BTB/POZ domain zinc finger isoforms. Association with differentiation of hippocampal neurons, cerebellar granule cells, and macroglia.  |  Mitchelmore, C., et al. 2002. J Biol Chem. 277: 7598-609. PMID: 11744704
  4. Hippocampus-like corticoneurogenesis induced by two isoforms of the BTB-zinc finger gene Zbtb20 in mice.  |  Nielsen, JV., et al. 2007. Development. 134: 1133-40. PMID: 17301088
  5. Zinc finger protein ZBTB20 is a key repressor of alpha-fetoprotein gene transcription in liver.  |  Xie, Z., et al. 2008. Proc Natl Acad Sci U S A. 105: 10859-64. PMID: 18669658
  6. Zinc finger protein Zbtb20 is essential for postnatal survival and glucose homeostasis.  |  Sutherland, AP., et al. 2009. Mol Cell Biol. 29: 2804-15. PMID: 19273596
  7. Zbtb20 promotes astrocytogenesis during neocortical development.  |  Nagao, M., et al. 2016. Nat Commun. 7: 11102. PMID: 27000654
  8. ZBTB20 regulates EGFR expression and hepatocyte proliferation in mouse liver regeneration.  |  Zhang, H., et al. 2018. Cell Death Dis. 9: 462. PMID: 29700307
  9. Zbtb20 Regulates Developmental Neurogenesis in the Olfactory Bulb and Gliogenesis After Adult Brain Injury.  |  Doeppner, TR., et al. 2019. Mol Neurobiol. 56: 567-582. PMID: 29748916
  10. Primrose syndrome: a phenotypic comparison of patients with a ZBTB20 missense variant versus a 3q13.31 microdeletion including ZBTB20.  |  Juven, A., et al. 2020. Eur J Hum Genet. 28: 1044-1055. PMID: 32071410
  11. ZBTB20 is crucial for the specification of a subset of callosal projection neurons and astrocytes in the mammalian neocortex.  |  Medeiros de Araújo, JA., et al. 2021. Development. 148: PMID: 34351428
  12. ZBTB20 suppresses tumor growth in glioblastoma through activating the TET1/FAS/caspase‑3 pathway.  |  Duan, P., et al. 2024. Oncol Lett. 28: 358. PMID: 38881713

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

ZBTB20 Antibody (E-11)

sc-515370
200 µg/ml
$316.00

ZBTB20 Antibody (E-11): m-IgG Fc BP-HRP Bundle

sc-528030
200 µg Ab; 10 µg BP
$354.00

ZBTB20 Antibody (E-11): m-IgG1 BP-HRP Bundle

sc-533403
200 µg Ab; 20 µg BP
$354.00

ZBTB20 Antibody (E-11) X

sc-515370 X
200 µg/0.1 ml
$316.00

ZBTB20 Antibody (E-11) AC

sc-515370 AC
500 µg/ml, 25% agarose
$416.00

ZBTB20 Antibody (E-11) HRP

sc-515370 HRP
200 µg/ml
$316.00

ZBTB20 Antibody (E-11) FITC

sc-515370 FITC
200 µg/ml
$330.00

ZBTB20 Antibody (E-11) PE

sc-515370 PE
200 µg/ml
$343.00

ZBTB20 Antibody (E-11) Alexa Fluor® 488

sc-515370 AF488
200 µg/ml
$357.00

ZBTB20 Antibody (E-11) Alexa Fluor® 546

sc-515370 AF546
200 µg/ml
$357.00

ZBTB20 Antibody (E-11) Alexa Fluor® 594

sc-515370 AF594
200 µg/ml
$357.00

ZBTB20 Antibody (E-11) Alexa Fluor® 647

sc-515370 AF647
200 µg/ml
$357.00

ZBTB20 Antibody (E-11) Alexa Fluor® 680

sc-515370 AF680
200 µg/ml
$357.00

ZBTB20 Antibody (E-11) Alexa Fluor® 790

sc-515370 AF790
200 µg/ml
$357.00

For Western Blot, is it recommended to use denatured or non-denatured conditions with ZBTB20 (E-11): sc-515370 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
  • y_2025, m_10, d_14, h_6CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_515370, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 111ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good for Western BlotAntibody detects ZBTB20 in HeLa and Jurkat nuclear extracts, HL-60, 3611-RF, and Jurkat cell lysates by Western blot -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from Clean in ImmunoHistologyTried the Sample test for Fluorescence ImmunoHistology -Brain perfused with 4% PFA, transcardially -non-embedded free-floting section (40um) -"Antigene retrieval" at 80° in 10mM NatriumCitrate -PBS buffer -Concentration 1:100---Not tried for WB
Date published: 2018-01-04
  • y_2025, m_10, d_14, h_6
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_515370, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
ZBTB20 Antibody (E-11) is rated 5.0 out of 5 by 2.
  • y_2025, m_10, d_14, h_6
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_515370, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 102ms
  • REVIEWS, PRODUCT