Z-DEVD-AFC
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Z-DEVD-AFC is a fluorogenic substrate that is used to measure caspase-3 activity in cell lysates. Upon cleavage by caspase-3, the AFC fluorophore is released, resulting in an increase in fluorescence that can be quantified using a fluorometer. This allows monitoring of the activation of caspase-3, a key executioner caspase involved in apoptosis, in response to various stimuli or experimental conditions. The cleavage of Z-DEVD-AFC by caspase-3 occurs at the Asp-Glu-Val-Asp (DEVD) sequence, making it useful for studying the molecular mechanisms of apoptosis. By providing a sensitive and quantitative readout of caspase-3 activity, Z-DEVD-AFC enables the investigation and regulation of apoptosis and the potential involvement of caspase-3 in various cellular processes.
Z-DEVD-AFC References
- Induction of apoptosis by a novel copper-based anticancer compound, casiopeina II, in L1210 murine leukaemia and CH1 human ovarian carcinoma cells. | De Vizcaya-Ruiz, A., et al. 2000. Toxicol In Vitro. 14: 1-5. PMID: 10699355
- Involvement of caspase 3 in apoptotic death of cortical neurons evoked by DNA damage. | Keramaris, E., et al. 2000. Mol Cell Neurosci. 15: 368-79. PMID: 10845773
- Disparate cleavage of poly-(ADP-ribose)-polymerase (PARP) and a synthetic tetrapeptide, DEVD, by apoptotic cells. | Yu, K., et al. 2001. Apoptosis. 6: 151-60. PMID: 11388664
- Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. | Nicholson, DW., et al. 1995. Nature. 376: 37-43. PMID: 7596430
- Cytotoxic T-cell-derived granzyme B activates the apoptotic protease ICE-LAP3. | Chinnaiyan, AM., et al. 1996. Curr Biol. 6: 897-9. PMID: 8805307
- The release of cytochrome c from mitochondria: a primary site for Bcl-2 regulation of apoptosis. | Kluck, RM., et al. 1997. Science. 275: 1132-6. PMID: 9027315
- Substrate specificities of caspase family proteases. | Talanian, RV., et al. 1997. J Biol Chem. 272: 9677-82. PMID: 9092497
- Processing/activation of CPP32-like proteases is involved in transforming growth factor beta1-induced apoptosis in rat hepatocytes. | Inayat-Hussain, SH., et al. 1997. Hepatology. 25: 1516-26. PMID: 9185777
- Caspase-7 is activated during lovastatin-induced apoptosis of the prostate cancer cell line LNCaP. | Marcelli, M., et al. 1998. Cancer Res. 58: 76-83. PMID: 9426061
- Caspase-mediated cleavage of the ubiquitin-protein ligase Nedd4 during apoptosis. | Harvey, KF., et al. 1998. J Biol Chem. 273: 13524-30. PMID: 9593687
- Fas-mediated apoptosis in mouse hepatocytes involves the processing and activation of caspases. | Jones, RA., et al. 1998. Hepatology. 27: 1632-42. PMID: 9620337
- Caspases: enemies within. | Thornberry, NA. and Lazebnik, Y. 1998. Science. 281: 1312-6. PMID: 9721091
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Z-DEVD-AFC, 5 mg | sc-296746 | 5 mg | $148.00 | |||
Z-DEVD-AFC, 50 mg | sc-296746A | 50 mg | $1331.00 |