MW: 821.71
A fluorogenic substrate for caspase-3.


  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 104ms
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
Synonym: Caspase 3 Apopain Substrate; Fluorogenic substrate for Caspase-3; Z-Asp-Glu-Val-Asp-7-Amino-4-trifluoromethylcoumarin
Application: A fluorogenic substrate for caspase-3
Molecular Weight: 821.71
Molecular Formula: C36H38F3N5O14
* Refer to Certificate of Analysis for lot specific data (including water content).

Z-DEVD-AFC is a fluorogenic substrate for caspase-3. It also acts as a substrate for granzyme B-processed ICE-LAP3, caspase-6, caspase-7, caspase-8, and caspase-10. The reaction can be observed by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.


1. Keramaris, E., et al. 2000. Mol. Cell Neurosci. 15: 368. PMID: 10845773
2. Thornberry, N.A., and Lazebnik, Y. 1998. Science. 281: 1312-1316.P MID: 9721091
3. Kluck, R.M., et al. 1997. Science. 275: 1132-1136. PMID: 9027315
4. Talanian, R.V., et al. 1997. J. Biol. Chem. 272: 9677-9682. PMID: 9092497
5. Chinnaiyan, A.M., et al. 1996. Curr. Biol. 6: 897-899. PMID: 8805307
6. Nicholson, D.W., et al. 1995. Nature. 376: 37-43. PMID: 7596430

Physical State :
Solubility :
Soluble in DMSO (10 mg/mL), and water.
Storage :
Store at -20° C
Fluoresence :
λex ~400 nm, λem ~505 nm; λex 370-390 nm, λem 500-510 nm; λex 380 nm, λem 500 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
CC(C)[[email protected]@H](C(=O)N[[email protected]@H](CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)[[email protected]](CCC(=O)O)NC(=O)[[email protected]](CC(=O)O)NC(=O)OCC3=CC=CC=C3

Download SDS (MSDS)

Certificate of Analysis

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 111ms
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 20ms
  • bvseo-msg: The resource to the URL or file is currently unavailable.;

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.