Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
YB-1 Double Nickase Plasmid (h): sc-401385-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- YB-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- YB-1 Double Nickase Plasmid (h) and YB-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting YBX1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: YB-1 Antibody (C-3): sc-398340
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
YB-1 Double Nickase Plasmid (h) | sc-401385-NIC | 20 µg | $410.00 | |||
YB-1 Double Nickase Plasmid (h2) | sc-401385-NIC-2 | 20 µg | $410.00 |
YBX1 encodes Y-box binding protein 1 (YB-1), a multifunctional DNA/RNA-binding factor that coordinates transcriptional regulation, mRNA splicing, stability, and translation. YB-1 participates in cellular stress responses and signal transduction networks, including pathways linked to MAPK/ERK and PI3K/AKT activity, and modulates expression of genes involved in proliferation, survival, and epithelial–mesenchymal plasticity. Through its roles in chromatin-associated transcriptional control and ribonucleoprotein complexes, YB-1 influences cell cycle progression, DNA damage responses, and adaptive stress programs. Dysregulated YBX1 expression or YB-1 phosphorylation has been associated with tumor biology, drug resistance phenotypes, and inflammatory signaling, making it a useful node for mechanistic studies in cancer and cell stress research.
YB-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the YBX1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within YBX1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt YBX1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of YBX1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.