YAP Double Nickase Plasmid (h): sc-400040-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- YAP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- YAP Double Nickase Plasmid (h) and YAP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting YAP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: YAP Antibody (G-6): sc-376830
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
YAP Double Nickase Plasmid (h) | sc-400040-NIC | 20 µg | $410.00 | |||
YAP Double Nickase Plasmid (h2) | sc-400040-NIC-2 | 20 µg | $410.00 |
YAP1 encodes the transcriptional co-activator YAP, a central effector of the Hippo signaling pathway that integrates cell density, mechanical cues, and polarity to control proliferation, apoptosis, and organ size. When Hippo kinases are inactive, YAP accumulates in the nucleus and partners with TEAD family transcription factors to regulate gene programs linked to growth, cytoskeletal remodeling, and extracellular matrix signaling. Dysregulated YAP activity is implicated in altered contact inhibition, epithelial–mesenchymal dynamics, and stem-like phenotypes, and is frequently studied in cancer biology, fibrosis, and regenerative processes. YAP also interfaces with GPCR, Wnt/β-catenin, and TGF-β signaling, making YAP1 a useful node for mapping pathway crosstalk and transcriptional network rewiring.
YAP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the YAP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within YAP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt YAP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of YAP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.