YAP Antibody (63.7): sc-101199

YAP Antibody (63.7) is a mouse monoclonal IgG2a kappa light chain antibody specifically designed to detect Yes-associated protein 1 (YAP1), also known simply as YAP, across multiple species including mouse, rat, and human. YAP monoclonal antibody (63.7) is raised against recombinant human YAP and is versatile in its application, effectively performing in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). YAP plays a pivotal role in the Hippo signaling pathway, acting as a transcriptional co-activator that regulates cell proliferation, apoptosis, and organ size, making YAP crucial for maintaining tissue homeostasis and implicated in various cancers when dysregulated. By interacting with 14-3-3 proteins through its phosphorylated serine residue (Ser 112), YAP is controlled in localization and activity, linking extracellular signals to gene expression in the nucleus. This regulation is essential for processes such as differentiation, cell cycle progression, and apoptosis, highlighting YAP′s importance in cellular function and development. Anti-YAP antibody (63.7) provides reliable detection of YAP′s expression levels and localization, facilitating research into YAP mechanisms and role in disease. With recognition capabilities across different species and multiple assay formats, YAP (63.7) antibody serves as an indispensable tool for scientists studying intricate YAP pathways. Additionally, YAP shares homology with TAZ (transcriptional co-activator with PDZ-binding motif), another key player in the Hippo pathway, further emphasizing YAP (63.7) monoclonal antibody′s relevance in comprehensive signaling studies. Expressed abundantly in tissues such as the lung, placenta, prostate, ovary, and testis, anti-YAP antibody (63.7) is essential for elucidating the diverse functions and regulatory mechanisms of YAP in both normal physiology and pathological conditions.

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YAP Antibody (63.7) References:

1. Akt phosphorylates the Yes-associated protein, YAP, to induce interaction with 14-3-3 and attenuation of p73-mediated apoptosis.  |  Basu, S., et al. 2003. Mol Cell. 11: 11-23. PMID: 12535517
2. Yes-associated protein (YAP) is a critical mediator of c-Jun-dependent apoptosis.  |  Danovi, SA., et al. 2008. Cell Death Differ. 15: 217-9. PMID: 17823615
3. Tumor suppressor LATS1 is a negative regulator of oncogene YAP.  |  Hao, Y., et al. 2008. J Biol Chem. 283: 5496-509. PMID: 18158288
4. Negative regulation of YAP by LATS1 underscores evolutionary conservation of the Drosophila Hippo pathway.  |  Zhang, J., et al. 2008. Cancer Res. 68: 2789-94. PMID: 18413746
5. Yes-associated protein (YAP) functions as a tumor suppressor in breast.  |  Yuan, M., et al. 2008. Cell Death Differ. 15: 1752-9. PMID: 18617895
6. Mst2 and Lats kinases regulate apoptotic function of Yes kinase-associated protein (YAP).  |  Oka, T., et al. 2008. J Biol Chem. 283: 27534-27546. PMID: 18640976
7. YAP1 is involved in mesothelioma development and negatively regulated by Merlin through phosphorylation.  |  Yokoyama, T., et al. 2008. Carcinogenesis. 29: 2139-46. PMID: 18725387
8. The neurofibromatosis 2 tumor suppressor gene product, merlin, regulates human meningioma cell growth by signaling through YAP.  |  Striedinger, K., et al. 2008. Neoplasia. 10: 1204-12. PMID: 18953429
9. Characterization of the mammalian YAP (Yes-associated protein) gene and its role in defining a novel protein module, the WW domain.  |  Sudol, M., et al. 1995. J Biol Chem. 270: 14733-41. PMID: 7782338

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-YAP Antibody (63.7) has 606 citations in a variety of scientific publications.

Primary antibodies like Anti-YAP Antibody (63.7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

YAP Antibody (63.7) Specifications

Alternate Product Name: YAP

Product Clone Name: 63.7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 53-162

Epitope Target: YAP

Epitope Species: human

Product Reactivity/Detection: YAP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 606 citations in various scientific publications

Available Conjugates/Forms for YAP Antibody (63.7): N/A

The preferred YAP antibody is YAP Antibody (H-9): sc-271134.