XRCC4 Antibody (G-10): sc-365118
- XRCC4 Antibody (G-10) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 307-337 at the C-terminus of XRCC4 of human origin
- Anti-XRCC4 Antibody (G-10) is recommended for detection of XRCC4 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-XRCC4 Antibody (G-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for XRCC4 Antibody (G-10) for WB applications. These reagents are now offered in bundles with XRCC4 Antibody (G-10) (see ordering information below).
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XRCC4 Antibody (G-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects XRCC4 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). XRCC4 (G-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The x-ray repair cross-complementing (XRCC) proteins play a crucial role in the repair and maintenance of genetic stability following DNA damage, with XRCC4 serving as an essential accessory factor for DNA ligase IV. XRCC4 preferentially binds to DNA with nicks or broken ends, enhancing the ligase′s activity in joining DNA strands, which is vital for the repair of DNA double-strand breaks. Additionally, XRCC4 is involved in V(D)J recombination, a process critical for the development of T and B cells in the immune system. Defects in XRCC4 or other components of the DNA repair machinery can lead to severe immunodeficiencies due to the inability to properly rearrange immunoglobulin and T-cell receptor genes, highlighting the importance of XRCC4 in both DNA repair and immune function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
XRCC4 Antibody (G-10) References:
- Electron microscopy of Xrcc4 and the DNA ligase IV-Xrcc4 DNA repair complex. | Recuero-Checa, MA., et al. 2009. DNA Repair (Amst). 8: 1380-9. PMID: 19837014
- DNA Ligase IV regulates XRCC4 nuclear localization. | Francis, DB., et al. 2014. DNA Repair (Amst). 21: 36-42. PMID: 24984242
- Ionizing radiation-induced XRCC4 phosphorylation is mediated through ATM in addition to DNA-PK. | Imamichi, S., et al. 2014. Proc Jpn Acad Ser B Phys Biol Sci. 90: 365-72. PMID: 25391321
- Lysine 271 but not lysine 210 of XRCC4 is required for the nuclear localization of XRCC4 and DNA ligase IV. | Fukuchi, M., et al. 2015. Biochem Biophys Res Commun. 461: 687-94. PMID: 25934149
- XRCC4/XLF Interaction Is Variably Required for DNA Repair and Is Not Required for Ligase IV Stimulation. | Roy, S., et al. 2015. Mol Cell Biol. 35: 3017-28. PMID: 26100018
- Absence of XRCC4 and its paralogs in human cells reveal differences in outcomes for DNA repair and V(D)J recombination. | Ruis, B., et al. 2020. DNA Repair (Amst). 85: 102738. PMID: 31731258
- An XRCC4 mutant mouse, a model for human X4 syndrome, reveals interplays with Xlf, PAXX, and ATM in lymphoid development. | Roch, B., et al. 2021. Elife. 10: PMID: 34519267
- XRCC4 and MRE11 Roles and Transcriptional Response to Repair of TALEN-Induced Double-Strand DNA Breaks. | Benjamin, R., et al. 2022. Int J Mol Sci. 23: PMID: 35054780
- Platinum-Resistant Ovarian Cancer Is Vulnerable to the cJUN-XRCC4 Pathway Inhibition. | Xu, M., et al. 2022. Cancers (Basel). 14: PMID: 36551554
- The XRCC4 gene product is a target for and interacts with the DNA-dependent protein kinase. | Leber, R., et al. 1998. J Biol Chem. 273: 1794-801. PMID: 9430729
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XRCC4 Antibody (G-10) | sc-365118 | 200 µg/ml | $322.00 | |||
XRCC4 Antibody (G-10): m-IgG Fc BP-HRP Bundle | sc-527334 | 200 µg Ab; 10 µg BP | $361.00 | |||
XRCC4 Antibody (G-10): m-IgG1 BP-HRP Bundle | sc-532707 | 200 µg Ab; 20 µg BP | $361.00 | |||
XRCC4 Antibody (G-10) AC | sc-365118 AC | 500 µg/ml, 25% agarose | $424.00 | |||
XRCC4 Antibody (G-10) HRP | sc-365118 HRP | 200 µg/ml | $322.00 | |||
XRCC4 Antibody (G-10) FITC | sc-365118 FITC | 200 µg/ml | $336.00 | |||
XRCC4 Antibody (G-10) PE | sc-365118 PE | 200 µg/ml | $349.00 | |||
XRCC4 Antibody (G-10) Alexa Fluor® 488 | sc-365118 AF488 | 200 µg/ml | $364.00 | |||
XRCC4 Antibody (G-10) Alexa Fluor® 546 | sc-365118 AF546 | 200 µg/ml | $364.00 | |||
XRCC4 Antibody (G-10) Alexa Fluor® 594 | sc-365118 AF594 | 200 µg/ml | $364.00 | |||
XRCC4 Antibody (G-10) Alexa Fluor® 647 | sc-365118 AF647 | 200 µg/ml | $364.00 | |||
XRCC4 Antibody (G-10) Alexa Fluor® 680 | sc-365118 AF680 | 200 µg/ml | $364.00 | |||
XRCC4 Antibody (G-10) Alexa Fluor® 790 | sc-365118 AF790 | 200 µg/ml | $364.00 | |||
XRCC4 (G-10) Neutralizing Peptide | sc-365118 P | 100 µg/0.5 ml | $69.00 |