Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
XPG Double Nickase Plasmid (h): sc-416940-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- XPG Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- XPG Double Nickase Plasmid (h) and XPG Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ERCC5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: XPG Antibody (8H7): sc-13563
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XPG Double Nickase Plasmid (h) | sc-416940-NIC | 20 µg | $410.00 |
ERCC5 encodes the endonuclease XPG, a structure-specific nuclease essential for nucleotide excision repair (NER) that cleaves damaged DNA on the 3′ side of lesion-containing bubbles. XPG operates within the TFIIH-centered repair complex and supports genome stability by resolving UV photoproducts and bulky adducts, thereby limiting replication stress and mutation accumulation. In addition to canonical NER, XPG has been linked to transcription-coupled repair and processing of R-loops and other DNA secondary structures that intersect with ATR/CHK1 signaling. Pathogenic ERCC5 dysfunction is associated with disorders marked by impaired DNA damage responses and accelerated cellular decline, making it a key node for studying genotype–phenotype relationships in DNA repair biology.
XPG Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ERCC5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ERCC5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ERCC5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ERCC5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.