XBP1 Double Nickase Plasmid (h): sc-400131-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- XBP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- XBP1 Double Nickase Plasmid (h) and XBP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting XBP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: XBP1 Antibody (F-4): sc-8015
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XBP1 Double Nickase Plasmid (h) | sc-400131-NIC | 20 µg | $410.00 | |||
XBP1 Double Nickase Plasmid (h2) | sc-400131-NIC-2 | 20 µg | $410.00 |
XBP1 (X-box binding protein 1) is a basic leucine zipper transcription factor that serves as a central effector of the unfolded protein response, particularly downstream of ER stress signaling through IRE1-mediated unconventional splicing. The spliced XBP1 isoform drives transcriptional programs that expand ER capacity, promote protein folding and secretion, and coordinate proteostasis with lipid metabolism and cellular differentiation. XBP1 activity is tightly linked to secretory cell function in immune and endocrine lineages and influences inflammatory signaling and metabolic adaptation under stress. Dysregulation of XBP1-dependent pathways has been associated with conditions involving chronic ER stress, including cancer biology, metabolic disease mechanisms, and immune-mediated disorders, making it a widely used node for studying stress-adaptation circuitry.
XBP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the XBP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within XBP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt XBP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of XBP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.