Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
WTAP CRISPR/Cas9 KO Plasmid (m): sc-425635
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WTAP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the WTAP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: WTAP Antibody (D-7): sc-374280
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WTAP CRISPR/Cas9 KO Plasmid (m) | sc-425635 | 20 µg | $397.00 |
Overview
Wtap encodes WTAP (Wilms tumor 1–associating protein), a core regulatory component of the mRNA N6-methyladenosine (m6A) writer complex that supports METTL3/METTL14 catalytic activity and coordinates nuclear localization of methylation machinery. Through m6A-dependent control of pre-mRNA processing, alternative splicing, transcript stability, and translation, WTAP influences cell-cycle progression, lineage specification, and stress-responsive gene expression programs. WTAP activity interfaces with RNA metabolism pathways in nuclear speckles and links epitranscriptomic regulation to developmental signaling and differentiation networks. Dysregulated WTAP-associated m6A deposition has been implicated in altered proliferation and differentiation states relevant to cancer biology and hematopoietic and developmental phenotypes in model systems.
WTAP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Wtap gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Wtap together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Wtap open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish WTAP protein expression.
This CRISPR knockout system enables efficient generation of Wtap-deficient cell models for investigation of WTAP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Wtap exon(s) critical for WTAP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Wtap genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by WTAP CRISPR/Cas9 KO Plasmid (m) and WTAP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Wtap locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by WTAP HDR Plasmid (m) and WTAP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Wtap homology arms to support homology-directed repair at defined Wtap target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.