Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
WTAP Antibody (D-7): sc-374280. Western blot analysis of WTAP expression in K-562 nuclear extract (A) and THP-1 (B) and NIH/3T3 (C) whole cell lysates.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
WTAP CRISPR/Cas9 KO Plasmid (m): sc-425635
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WTAP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the WTAP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: WTAP Antibody (D-7): sc-374280
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WTAP CRISPR/Cas9 KO Plasmid (m) | sc-425635 | 20 µg | $397.00 |