WSB1 Double Nickase Plasmid (m): sc-429733-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WSB1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- WSB1 Double Nickase Plasmid (m) and WSB1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Wsb1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: WSB1 Antibody (A-10): sc-393483
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WSB1 Double Nickase Plasmid (m) | sc-429733-NIC | 20 µg | $410.00 |
Wsb1 encodes the mouse WD repeat and SOCS box–containing protein 1 (WSB1), an adaptor component of ECS-type E3 ubiquitin ligase complexes that helps couple substrate recognition to ubiquitin-dependent proteostasis. Through modulation of protein turnover, WSB1 has been linked to regulation of signaling outputs such as hypoxia-responsive pathways and cellular stress adaptation, with downstream effects on proliferation and differentiation programs. Altered WSB1 activity has been associated in the literature with dysregulated ubiquitination networks that intersect with oncogenic signaling and tissue homeostasis. These properties make Wsb1 a useful target for studying ubiquitin pathway control of signal transduction and disease-relevant phenotypes in mouse model systems.
WSB1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Wsb1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Wsb1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Wsb1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Wsb1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.