WSB1 Double Nickase Plasmid (h): sc-411879-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WSB1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- WSB1 Double Nickase Plasmid (h) and WSB1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting WSB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: WSB1 Antibody (A-10): sc-393483
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WSB1 Double Nickase Plasmid (h) | sc-411879-NIC | 20 µg | $410.00 | |||
WSB1 Double Nickase Plasmid (h2) | sc-411879-NIC-2 | 20 µg | $410.00 |
WSB1 (WD repeat and SOCS box containing 1) encodes a substrate-recognition component of Cullin-RING E3 ubiquitin ligase complexes, linking specific protein targets to ubiquitination and proteasomal turnover. Through its WD-repeat domain and SOCS box, WSB1 helps coordinate protein quality control and signal-dependent remodeling of cellular proteomes, with downstream effects on stress responses, growth factor signaling, and transcriptional programs. Altered WSB1 activity has been investigated in contexts where ubiquitin-mediated regulation contributes to disease-associated phenotypes, including tumor biology and dysregulated cellular adaptation to hypoxia and stress. As a node in ubiquitin pathway circuitry, WSB1 is frequently studied for its impact on pathway dynamics and proteostasis-dependent regulation.
WSB1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the WSB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within WSB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt WSB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of WSB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.