WRP Antibody (E-11): sc-374503

WRP Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects WRP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). WRP (E-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The WAVE-associated Rac GTPase-activating protein (WRP), also known as SLIT-ROBO Rho GTPase-activating protein 3 (srGAP3) and Mental disorder-activating GAP (MEGAP), is a 1099 amino acid protein characterized by the presence of one FCH domain, one Rho-GAP domain, and one SH3 domain. WRP is predominantly expressed in the brain, with lower expression levels found in the kidney, and plays a crucial role in regulating cell migration through interactions with signaling proteins Cdc42 and Rac1. By downregulating Cdc42 and Rac1 activity, WRP impairs actin and microtubule dynamics, which are essential for cellular protrusion formation and overall cell migration. Defects in the gene encoding WRP have been associated with severe idiopathic mental retardation, highlighting WRP′s significance in neurodevelopmental processes. Three isoforms of WRP arise from alternative splicing events, which may contribute to functional diversity in various cellular contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

WRP Antibody (E-11) References:

1. Signal transduction in neuronal migration: roles of GTPase activating proteins and the small GTPase Cdc42 in the Slit-Robo pathway.  |  Wong, K., et al. 2001. Cell. 107: 209-21. PMID: 11672528
2. The novel Rho-GTPase activating gene MEGAP/ srGAP3 has a putative role in severe mental retardation.  |  Endris, V., et al. 2002. Proc Natl Acad Sci U S A. 99: 11754-9. PMID: 12195014
3. The WRP component of the WAVE-1 complex attenuates Rac-mediated signalling.  |  Soderling, SH., et al. 2002. Nat Cell Biol. 4: 970-5. PMID: 12447388
4. Regulation of actin dynamics by WASP family proteins.  |  Miki, H. and Takenawa, T. 2003. J Biochem. 134: 309-13. PMID: 14561714
5. A WAVE-1 and WRP signaling complex regulates spine density, synaptic plasticity, and memory.  |  Soderling, SH., et al. 2007. J Neurosci. 27: 355-65. PMID: 17215396
6. Expression of MEGAP mRNA during embryonic development.  |  Waltereit, R., et al. 2008. Gene Expr Patterns. 8: 307-10. PMID: 18343202
7. Dynamic expression of the Slit-Robo GTPase activating protein genes during development of the murine nervous system.  |  Bacon, C., et al. 2009. J Comp Neurol. 513: 224-36. PMID: 19137586
8. WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac.  |  Miki, H., et al. 1998. EMBO J. 17: 6932-41. PMID: 9843499

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-WRP Antibody (E-11) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-WRP Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

WRP Antibody (E-11) Specifications

Alternate Product Name: WRP

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 821-970

Epitope Target: WRP

Epitope Species: human

Product Reactivity/Detection: WRP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for WRP Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin