Wnt-6 CRISPR/Cas9 KO Plasmid (m): sc-423720
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Wnt-6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Wnt-6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Wnt-6 CRISPR/Cas9 KO Plasmid (m) | sc-423720 | 20 µg | $397.00 |
Overview
Wnt6 encodes Wnt-6, a secreted glycoprotein that functions as a short-range morphogen in Wnt signaling to regulate cell fate specification, proliferation, polarity, and tissue patterning during embryogenesis and adult homeostasis. In mouse systems, Wnt-6 can engage Frizzled/LRP receptor complexes to modulate canonical β-catenin–dependent transcription as well as non-canonical pathways that influence cytoskeletal remodeling and directional migration. Altered Wnt6 activity perturbs developmental programs and epithelial–mesenchymal signaling, processes frequently studied in organogenesis, regeneration, and cancer biology models. Dysregulated Wnt signaling is broadly implicated in congenital defects and tumor-associated pathway rewiring, making Wnt6 a relevant node for mechanistic pathway interrogation.
Wnt-6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Wnt6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Wnt6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Wnt6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Wnt-6 protein expression.
This CRISPR knockout system enables efficient generation of Wnt6-deficient cell models for investigation of Wnt-6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Wnt6 exon(s) critical for Wnt-6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Wnt6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Wnt-6 CRISPR/Cas9 KO Plasmid (m) and Wnt-6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Wnt6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Wnt-6 HDR Plasmid (m) and Wnt-6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Wnt6 homology arms to support homology-directed repair at defined Wnt6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.