Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Wnt-4 CRISPR/Cas9 KO Plasmid (m): sc-423717
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Wnt-4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Wnt-4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Wnt-4 Antibody (B-6): sc-376279
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Wnt-4 CRISPR/Cas9 KO Plasmid (m) | sc-423717 | 20 µg | $397.00 |
Overview
Wnt4 encodes the secreted signaling ligand Wnt-4, a key regulator of WNT pathway activity that shapes embryonic development and adult tissue homeostasis. In mouse, Wnt-4 influences cell fate decisions, polarity, migration, and differentiation through context-dependent activation of canonical β-catenin signaling and non-canonical WNT pathways. It is strongly linked to reproductive tract and kidney morphogenesis, osteogenic and stromal programs, and immune microenvironment modulation via downstream transcriptional and cytoskeletal remodeling networks. Dysregulated Wnt-4 signaling has been associated with developmental phenotypes and with disease-relevant processes such as fibrosis, aberrant differentiation, and tumor-associated pathway rewiring used as mechanistic readouts in biomedical research models.
Wnt-4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Wnt4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Wnt4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Wnt4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Wnt-4 protein expression.
This CRISPR knockout system enables efficient generation of Wnt4-deficient cell models for investigation of Wnt-4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Wnt4 exon(s) critical for Wnt-4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Wnt4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Wnt-4 CRISPR/Cas9 KO Plasmid (m) and Wnt-4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Wnt4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Wnt-4 HDR Plasmid (m) and Wnt-4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Wnt4 homology arms to support homology-directed repair at defined Wnt4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.