Wnt-3 CRISPR/Cas9 KO Plasmid (h): sc-401936
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Wnt-3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Wnt-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Wnt-3 CRISPR/Cas9 KO Plasmid (h) | sc-401936 | 20 µg | $397.00 |
Overview
WNT3 encodes the secreted glycoprotein Wnt-3, a canonical Wnt ligand that activates Frizzled/LRP receptors to stabilize β-catenin and modulate TCF/LEF-dependent transcription. This signaling controls embryonic patterning, stem and progenitor cell maintenance, and regulation of proliferation and differentiation programs across multiple tissues. Aberrant WNT3/Wnt-3 activity perturbs Wnt/β-catenin network output and intersects with pathways governing epithelial–mesenchymal dynamics, cell fate specification, and tissue homeostasis. Dysregulation of Wnt-3-associated signaling has been implicated in developmental abnormalities and disease-relevant transcriptional states, making it a useful target for mechanistic pathway studies.
Wnt-3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the WNT3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the WNT3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the WNT3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Wnt-3 protein expression.
This CRISPR knockout system enables efficient generation of WNT3-deficient cell models for investigation of Wnt-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting WNT3 exon(s) critical for Wnt-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple WNT3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Wnt-3 CRISPR/Cas9 KO Plasmid (h) and Wnt-3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the WNT3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Wnt-3 HDR Plasmid (h) and Wnt-3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by WNT3 homology arms to support homology-directed repair at defined WNT3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.