Wnt-2b Double Nickase Plasmid (h): sc-405478-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Wnt-2b Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Wnt-2b Double Nickase Plasmid (h) and Wnt-2b Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting WNT2B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Wnt-2b Antibody (C-2): sc-166502
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Wnt-2b Double Nickase Plasmid (h) | sc-405478-NIC | 20 µg | $410.00 | |||
Wnt-2b Double Nickase Plasmid (h2) | sc-405478-NIC-2 | 20 µg | $410.00 |
WNT2B encodes Wnt-2b, a secreted glycoprotein ligand that engages Frizzled/LRP receptors to regulate Wnt signaling during embryogenesis and adult tissue homeostasis. Through effects on β-catenin–dependent transcription and crosstalk with non-canonical Wnt pathways, Wnt-2b influences cell fate specification, proliferation, migration, and epithelial–mesenchymal programs. Altered WNT2B expression or signaling context has been linked to dysregulated developmental patterning and remodeling processes in epithelial and mesenchymal compartments, and is frequently studied in disease-relevant models where Wnt pathway activity shapes cell-state transitions. As a pathway node upstream of transcriptional programs controlling differentiation and tissue architecture, WNT2B is commonly interrogated to map ligand-driven signaling dynamics and receptor-ligand specificity.
Wnt-2b Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the WNT2B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within WNT2B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt WNT2B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of WNT2B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.