Wip1 Antibody (F-10): sc-376257

Wip1 Antibody (F-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Wip1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Wip1 antibody (F-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Wip1, also known as wildtype p53-induced phosphatase or PPM1D, plays a crucial role in the p53 DNA damage response pathway and is a member of the protein phosphatase 2C family. This serine/threonine protein phosphatase is essential for regulating cellular stress responses, as Wip1 selectively inactivates p38 MAPK and dephosphorylates key targets such as ATM/ATR, Chk1, and p53, thereby modulating cell cycle progression and apoptosis. Wip1 is ubiquitously expressed, with particularly high levels found in the testis, highlighting its importance in reproductive biology. Notably, Wip1 deletion has been shown to impair T and B cell function and hinder cell division, which contributes to a reduced risk of tumorigenesis, making Wip1 a significant factor in cancer research.

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Wip1 Antibody (F-10) References:

1. Subunit interactions control protein phosphatase 2A. Effects of limited proteolysis, N-ethylmaleimide, and heparin on the interaction of the B subunit.  |  Kamibayashi, C., et al. 1991. J Biol Chem. 266: 13251-60. PMID: 1649186
2. Wip1 phosphatase-deficient mice exhibit defective T cell maturation due to sustained p53 activation.  |  Schito, ML., et al. 2006. J Immunol. 176: 4818-25. PMID: 16585576
3. The Wip1 phosphatase (PPM1D) antagonizes activation of the Chk2 tumour suppressor kinase.  |  Oliva-Trastoy, M., et al. 2007. Oncogene. 26: 1449-58. PMID: 16936775
4. Regulation of ATM/p53-dependent suppression of myc-induced lymphomas by Wip1 phosphatase.  |  Shreeram, S., et al. 2006. J Exp Med. 203: 2793-9. PMID: 17158963
5. Protein phosphatases: recent progress.  |  Shenolikar, S. and Nairn, AC. 1991. Adv Second Messenger Phosphoprotein Res. 23: 1-121. PMID: 1847640
6. Protein serine/threonine phosphatases; an expanding family.  |  Cohen, PT., et al. 1990. FEBS Lett. 268: 355-9. PMID: 2166691
7. The structure and regulation of protein phosphatases.  |  Cohen, P. 1989. Annu Rev Biochem. 58: 453-508. PMID: 2549856
8. Wip1 inhibits neutrophil extracellular traps to promote abscess formation in mice by directly dephosphorylating Coronin-1a.  |  Chen, Y., et al. 2023. Cell Mol Immunol. 20: 941-954. PMID: 37386173
9. WIP1 is a novel specific target for growth hormone action.  |  Apaydin, T., et al. 2023. iScience. 26: 108117. PMID: 37876819
10. Targeting WIP1 phosphatase promotes partial remission in experimental collapsing glomerulopathy.  |  Duret, LC., et al. 2024. Kidney Int. 105: 980-996. PMID: 38423182
11. Protein phosphatases: properties and role in cellular regulation.  |  Ingebritsen, TS. and Cohen, P. 1983. Science. 221: 331-8. PMID: 6306765
12. Wip1, a novel human protein phosphatase that is induced in response to ionizing radiation in a p53-dependent manner.  |  Fiscella, M., et al. 1997. Proc Natl Acad Sci U S A. 94: 6048-53. PMID: 9177166

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Wip1 Antibody (F-10) has 33 citations in a variety of scientific publications.

Primary antibodies like Anti-Wip1 Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Wip1 Antibody (F-10) Specifications

Alternate Product Name: Wip1

Product Clone Name: F-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 306-605

Epitope Target: Wip1

Epitope Species: human

Product Reactivity/Detection: Wip1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 33 citations in various scientific publications

Available Conjugates/Forms for Wip1 Antibody (F-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin