Whirlin Antibody (G-8): sc-365250

Whirlin Antibody (G-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Whirlin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Whirlin antibody (G-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Whirlin is a cytoplasmic PDZ domain-containing protein crucial for elongation and maintenance of stereocilia, essential mechanosensory organelles found in hair cells of the inner ear. Whirlin co-localizes with actin filaments and is primarily localized in cochlear hair cells, playing a significant role in the dynamic Usher protein interactome. Whirlin interacts with Myosin XVa, a motor protein that binds to the second and third PDZ domains through its C-terminal PDZ-ligand, facilitating transport to stereocilia tips for elongation. The interaction with p55 is critical, as mutations in the DFNB31 gene, which encodes Whirlin, can lead to early loss of p55 labeling in stereocilia, potentially resulting in recessive hearing loss in both rats and humans.

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Whirlin Antibody (G-8) References:

1. Defects in whirlin, a PDZ domain molecule involved in stereocilia elongation, cause deafness in the whirler mouse and families with DFNB31.  |  Mburu, P., et al. 2003. Nat Genet. 34: 421-8. PMID: 12833159
2. Stereocilia: the long and the short of it.  |  Belyantseva, IA., et al. 2003. Trends Mol Med. 9: 458-61. PMID: 14604820
3. Myosin XVa and whirlin, two deafness gene products required for hair bundle growth, are located at the stereocilia tips and interact directly.  |  Delprat, B., et al. 2005. Hum Mol Genet. 14: 401-10. PMID: 15590698
4. Myosin-XVa is required for tip localization of whirlin and differential elongation of hair-cell stereocilia.  |  Belyantseva, IA., et al. 2005. Nat Cell Biol. 7: 148-56. PMID: 15654330
5. Identification of a novel frameshift mutation in the DFNB31/WHRN gene in a Tunisian consanguineous family with hereditary non-syndromic recessive hearing loss.  |  Tlili, A., et al. 2005. Hum Mutat. 25: 503. PMID: 15841483
6. Balanced levels of Espin are critical for stereociliary growth and length maintenance.  |  Rzadzinska, A., et al. 2005. Cell Motil Cytoskeleton. 62: 157-65. PMID: 16206170
7. Usherin, the defective protein in Usher syndrome type IIA, is likely to be a component of interstereocilia ankle links in the inner ear sensory cells.  |  Adato, A., et al. 2005. Hum Mol Genet. 14: 3921-32. PMID: 16301217
8. The DFNB31 gene product whirlin connects to the Usher protein network in the cochlea and retina by direct association with USH2A and VLGR1.  |  van Wijk, E., et al. 2006. Hum Mol Genet. 15: 751-65. PMID: 16434480
9. Whirlin complexes with p55 at the stereocilia tip during hair cell development.  |  Mburu, P., et al. 2006. Proc Natl Acad Sci U S A. 103: 10973-8. PMID: 16829577

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Whirlin Antibody (G-8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Whirlin Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Whirlin Antibody (G-8) Specifications

Alternate Product Name: Whirlin

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 723-751-

Epitope Target: Whirlin

Epitope Species: human

Product Reactivity/Detection: all isoforms of Whirlin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Whirlin Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin