Wee 1 CRISPR/Cas9 KO Plasmid (m): sc-423702
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Wee 1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Wee 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Wee 1 CRISPR/Cas9 KO Plasmid (m) | sc-423702 | 20 µg | $397.00 |
Overview
Mouse Wee1 encodes the WEE1 protein kinase, a central regulator of the G2/M checkpoint that restrains CDK1 activity through inhibitory phosphorylation to prevent premature mitotic entry. By integrating signals from ATR/CHK1-mediated DNA damage and replication stress pathways, WEE1 helps coordinate genome integrity with cell-cycle progression. Disruption of Wee1 can alter checkpoint control, replication stress tolerance, and mitotic fidelity, processes frequently implicated in tumor biology and other proliferative disorders. Wee1 is therefore widely studied in mechanisms of cell-cycle regulation, DNA damage responses, and synthetic lethal interactions with other checkpoint and repair factors.
Wee 1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Wee1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Wee1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Wee1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Wee 1 protein expression.
This CRISPR knockout system enables efficient generation of Wee1-deficient cell models for investigation of Wee 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Wee1 exon(s) critical for Wee 1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Wee1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Wee 1 CRISPR/Cas9 KO Plasmid (m) and Wee 1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Wee1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Wee 1 HDR Plasmid (m) and Wee 1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Wee1 homology arms to support homology-directed repair at defined Wee1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.