WDR96 CRISPR/Cas9 KO Plasmid (h): sc-407276
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WDR96 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the WDR96 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WDR96 CRISPR/Cas9 KO Plasmid (h) | sc-407276 | 20 µg | $397.00 |
Overview
CFAP43 encodes the human WD repeat–containing protein WDR96, a predicted scaffold involved in protein–protein interactions that support ciliary and flagellar architecture. WD-repeat proteins commonly organize multiprotein assemblies that regulate microtubule-based structures, and CFAP43 has been linked to processes underlying axonemal organization and motile cilia function. Disruption of cilia-associated components can alter cell polarity, fluid flow sensing, and intracellular signaling pathways coordinated at the centrosome/basal body. Genetic variation in CFAP43 has been associated with sperm flagellar defects and male infertility phenotypes, making it relevant for studies of ciliopathies and reproductive biology.
WDR96 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CFAP43 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CFAP43 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CFAP43 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish WDR96 protein expression.
This CRISPR knockout system enables efficient generation of CFAP43-deficient cell models for investigation of WDR96 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CFAP43 exon(s) critical for WDR96 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CFAP43 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by WDR96 CRISPR/Cas9 KO Plasmid (h) and WDR96 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CFAP43 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by WDR96 HDR Plasmid (h) and WDR96 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CFAP43 homology arms to support homology-directed repair at defined CFAP43 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.