WDR82 Lentiviral Activation Particles (h): sc-406963-LAC

WDR82 encodes a WD repeat–containing protein that functions as a chromatin-associated adaptor linking transcriptional programs to histone modification machinery. It is best characterized as a component of SETD1A/SETD1B COMPASS complexes that support H3K4 methylation and coordinate transcription initiation and RNA polymerase II–associated processes. Through these interactions, WDR82 contributes to promoter-proximal regulation, chromatin state maintenance, and gene expression fidelity during cell growth and differentiation. Dysregulation of COMPASS-associated factors and H3K4 methylation networks is frequently implicated in altered transcriptional control in cancer and neurodevelopmental disease, making WDR82 a useful node for mechanistic studies of epigenetic regulation.

WDR82 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient WDR82 upregulation across a broader range of human cell types.

WDR82 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the WDR82 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous WDR82 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native WDR82 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.