WDR77 Antibody (FG-4): sc-100899
- WDR77 Antibody (FG-4) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 100 µg/ml
- raised against recombinant WDR77 of human origin
- recommended for detection of WDR77 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for WDR77 Antibody (FG-4). This work is in progress.
WDR77 Antibody (FG-4) is a mouse monoclonal IgG1 antibody that detects WDR77 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). WDR77, also known as MEP50, is a 342 amino acid protein characterized by the presence of five WD-repeats, which are essential for its role in the early assembly of U small nuclear ribonucleoproteins (snRNPs). WDR77 is a critical component of a methyltransferase complex that includes protein arginine methyltransferase 5 (PRMT5), which catalyzes the conversion of arginine residues to dimethylarginines in spliceosomal Sm proteins. This post-translational modification facilitates the targeting of Sm proteins to the survival of motor neurons (SMN) complex, where they are incorporated into ribonucleoprotein core particles essential for RNA processing and splicing. Proper functioning of WDR77 and associated complexes is crucial for maintaining cellular homeostasis, and dysregulation has been implicated in the development of testicular tumors, highlighting potential roles in carcinogenesis. anti-WDR77 antibody (FG-4) is an invaluable tool for researchers investigating molecular mechanisms underlying RNA metabolism and implications in cancer biology.
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WDR77 Antibody (FG-4) References:
- P44/WDR77 restricts the sensitivity of proliferating cells to TGFβ signaling. | Yi, P., et al. 2014. Biochem Biophys Res Commun. 450: 409-15. PMID: 24944016
- MTAP deletion confers enhanced dependency on the PRMT5 arginine methyltransferase in cancer cells. | Kryukov, GV., et al. 2016. Science. 351: 1214-8. PMID: 26912360
- The PRMT5/WDR77 complex regulates alternative splicing through ZNF326 in breast cancer. | Rengasamy, M., et al. 2017. Nucleic Acids Res. 45: 11106-11120. PMID: 28977470
- Sirtuin 7-mediated deacetylation of WD repeat domain 77 (WDR77) suppresses cancer cell growth by reducing WDR77/PRMT5 transmethylase complex activity. | Qi, H., et al. 2018. J Biol Chem. 293: 17769-17779. PMID: 30282801
- WD Repeat Domain 77 Protein Regulates Translation of E2F1 and E2F3 mRNA. | Altayyar, MA., et al. 2020. Mol Cell Biol. 40: PMID: 33020149
- MafB, WDR77, and ß-catenin interact with each other and have similar genome association profiles. | He, L., et al. 2022. PLoS One. 17: e0264799. PMID: 35482762
- WDR77 inhibits prion-like aggregation of MAVS to limit antiviral innate immune response. | Li, J., et al. 2023. Nat Commun. 14: 4824. PMID: 37563140
- WDR77 in Pan-Cancer: Revealing expression patterns, genetic insights, and functional roles across diverse tumor types, with a spotlight on colorectal cancer. | Wang, Y., et al. 2024. Transl Oncol. 49: 102089. PMID: 39182364
- TBL2 Promotes Tumorigenesis via PRMT5/WDR77-Mediated AKT Activation in Breast Cancer. | Lu, X., et al. 2024. Adv Sci (Weinh). e2400160. PMID: 39499734
- PRMT5/WDR77 Enhances the Proliferation of Squamous Cell Carcinoma via the ΔNp63α-p21 Axis. | Liang, H., et al. 2024. Cancers (Basel). 16: PMID: 39594744
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WDR77 Antibody (FG-4) | sc-100899 | 100 µg/ml | $339.00 |