WDR59 CRISPR/Cas9 KO Plasmid (h): sc-405648
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WDR59 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the WDR59 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WDR59 CRISPR/Cas9 KO Plasmid (h) | sc-405648 | 20 µg | $397.00 |
Overview
WDR59 (WD repeat domain 59) is a WD40-repeat scaffolding protein that functions as a core component of the GATOR2 complex, a key upstream regulator of mTORC1 signaling. Through GATOR2, WDR59 contributes to amino acid–sensing pathways that control lysosomal mTORC1 activation, thereby influencing protein synthesis, nutrient-responsive growth programs, and autophagy. Perturbation of WDR59-dependent signaling can remodel cellular metabolism and stress responses, processes frequently interrogated in cancer biology and other disorders linked to dysregulated mTOR activity. As a consequence, WDR59 is routinely studied for its role in integrating nutrient availability with cell growth and homeostasis.
WDR59 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the WDR59 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the WDR59 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the WDR59 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish WDR59 protein expression.
This CRISPR knockout system enables efficient generation of WDR59-deficient cell models for investigation of WDR59 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting WDR59 exon(s) critical for WDR59 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple WDR59 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by WDR59 CRISPR/Cas9 KO Plasmid (h) and WDR59 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the WDR59 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by WDR59 HDR Plasmid (h) and WDR59 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by WDR59 homology arms to support homology-directed repair at defined WDR59 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.