WDR36 CRISPR/Cas9 KO Plasmid (h): sc-410298
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WDR36 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the WDR36 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WDR36 CRISPR/Cas9 KO Plasmid (h) | sc-410298 | 20 µg | $397.00 |
Overview
WDR36 (WD repeat domain 36) encodes a nucleolar WD-repeat protein implicated in ribosome biogenesis and pre-rRNA processing, supporting maturation of the 18S rRNA and assembly of the small ribosomal subunit. Through its role in nucleolar function and RNA metabolism, WDR36 influences proteostasis and cellular growth programs that depend on efficient translation. Genetic variation and altered WDR36 function have been associated with ocular phenotypes including primary open-angle glaucoma, motivating mechanistic studies in relevant cell types. Disruption of WDR36 can also be used to probe how perturbations in ribosome production engage nucleolar stress responses and downstream signaling.
WDR36 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the WDR36 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the WDR36 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the WDR36 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish WDR36 protein expression.
This CRISPR knockout system enables efficient generation of WDR36-deficient cell models for investigation of WDR36 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting WDR36 exon(s) critical for WDR36 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple WDR36 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by WDR36 CRISPR/Cas9 KO Plasmid (h) and WDR36 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the WDR36 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by WDR36 HDR Plasmid (h) and WDR36 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by WDR36 homology arms to support homology-directed repair at defined WDR36 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.