Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
WDR33 Double Nickase Plasmid (h): sc-409284-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WDR33 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- WDR33 Double Nickase Plasmid (h) and WDR33 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting WDR33. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: WDR33 Antibody (D-1): sc-374466
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WDR33 Double Nickase Plasmid (h) | sc-409284-NIC | 20 µg | $410.00 |
Human WDR33 encodes a core component of the cleavage and polyadenylation specificity factor (CPSF) complex that mediates 3′ end processing of pre-mRNAs. WDR33 participates in recognition of the canonical AAUAAA polyadenylation signal and helps coordinate endonucleolytic cleavage with poly(A) tail addition, shaping transcript stability, nuclear export, and translation. Through its role in alternative polyadenylation, WDR33 influences global gene expression programs and can affect cellular proliferation, differentiation, and stress responses. Dysregulation of 3′ end processing and polyadenylation pathway components, including WDR33, is relevant to studies of aberrant mRNA isoform usage and gene expression changes observed across diverse disease models.
WDR33 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the WDR33 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within WDR33. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt WDR33 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of WDR33-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.