WDR22 CRISPR/Cas9 KO Plasmid (m): sc-435826
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WDR22 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the WDR22 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WDR22 CRISPR/Cas9 KO Plasmid (m) | sc-435826 | 20 µg | $397.00 |
Overview
Dcaf5 encodes WDR22, a WD-repeat–containing protein implicated in DDB1–CUL4 E3 ubiquitin ligase biology, where WD-repeat adaptors help confer substrate specificity for ubiquitin-dependent proteostasis. Through these complexes, WDR22 is positioned to influence regulated protein turnover that coordinates cell-cycle progression, DNA replication/repair-associated quality control, and stress-responsive signaling. Altered ubiquitination dynamics are broadly relevant to mechanisms of genome instability and dysregulated proliferation, making Dcaf5/WDR22 a useful entry point for studying pathways linked to oncogenic transformation and other proliferative disorders in model systems. In mouse, genetic perturbation of Dcaf5 can help define tissue- and context-specific roles of DCAF-associated ubiquitin ligase networks.
WDR22 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Dcaf5 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Dcaf5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Dcaf5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish WDR22 protein expression.
This CRISPR knockout system enables efficient generation of Dcaf5-deficient cell models for investigation of WDR22 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Dcaf5 exon(s) critical for WDR22 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Dcaf5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by WDR22 CRISPR/Cas9 KO Plasmid (m) and WDR22 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Dcaf5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by WDR22 HDR Plasmid (m) and WDR22 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Dcaf5 homology arms to support homology-directed repair at defined Dcaf5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.