WDR17 CRISPR/Cas9 KO Plasmid (h): sc-414101
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WDR17 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the WDR17 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WDR17 CRISPR/Cas9 KO Plasmid (h) | sc-414101 | 20 µg | $397.00 |
Overview
WDR17 (WD repeat domain 17) encodes a predicted WD40 repeat–containing protein thought to function as a scaffold for multiprotein complex assembly, supporting regulated protein–protein interactions in the cytoplasm and at membrane-associated sites. Although its molecular partners remain incompletely defined, WD repeat proteins commonly influence intracellular trafficking, cytoskeletal organization, and signal integration by coordinating ubiquitination and other post-translational control mechanisms. Expression and genetic variation in WDR17 have been explored in the context of sensory and neurodevelopmental phenotypes, consistent with roles for WD-repeat scaffolds in specialized cell architecture and homeostatic pathways. These features make WDR17 a useful target for dissecting pathway wiring in human cells where scaffolding proteins modulate localization and stability of signaling components.
WDR17 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the WDR17 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the WDR17 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the WDR17 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish WDR17 protein expression.
This CRISPR knockout system enables efficient generation of WDR17-deficient cell models for investigation of WDR17 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting WDR17 exon(s) critical for WDR17 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple WDR17 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by WDR17 CRISPR/Cas9 KO Plasmid (h) and WDR17 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the WDR17 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by WDR17 HDR Plasmid (h) and WDR17 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by WDR17 homology arms to support homology-directed repair at defined WDR17 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.