WASP Antibody (B-9): sc-13139

WASP Antibody (B-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects WASP protein of mouse, rat, human, and mink origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-WASP antibody (B-9) is raised against amino acids 1-250 of WASP of human origin. WASP (B-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Wiskott-Aldrich syndrome (WAS) is a genetic disorder linked to a mutation on the short arm of the X chromosome, characterized by symptoms such as thrombocytopenia, eczema, immune system defects, and an increased risk of lymphoproliferative diseases. The gene responsible for WAS encodes WASP, a proline-rich protein that plays a crucial role in cytoskeletal organization. WASP is primarily located in the cytoplasm and is essential for the regulation of actin polymerization, which is vital for various cellular processes, including cell motility, shape, and division. WASP′s location in the cell is underscored by interaction with the Arp2/3 complex, which facilitates the nucleation of new actin filaments, thereby influencing the dynamics of the cytoskeleton. Disruption of WASP function can lead to severe immunological consequences, highlighting WASP′s critical role in maintaining cellular architecture and function.

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WASP Antibody (B-9) References:

1. Cdc42-interacting protein 4 promotes breast cancer cell invasion and formation of invadopodia through activation of N-WASp.  |  Pichot, CS., et al. 2010. Cancer Res. 70: 8347-56. PMID: 20940394
2. N-WASP promotes invasion and migration of cervical cancer cells through regulating p38 MAPKs signaling pathway.  |  Hou, J., et al. 2017. Am J Transl Res. 9: 403-415. PMID: 28337270
3. Wiskott-Aldrich syndrome protein (WASP) is a tumor suppressor in T cell lymphoma.  |  Menotti, M., et al. 2019. Nat Med. 25: 130-140. PMID: 30510251
4. The role of WASp in T cells and B cells.  |  Sun, X., et al. 2019. Cell Immunol. 341: 103919. PMID: 31047647
5. Macrophage mitochondrial fission improves cancer cell phagocytosis induced by therapeutic antibodies and is impaired by glutamine competition.  |  Li, J., et al. 2022. Nat Cancer. 3: 453-470. PMID: 35484420
6. Wiskott Aldrich syndrome protein (WASp)-deficient Th1 cells promote R-loop-driven transcriptional insufficiency and transcription-coupled nucleotide excision repair factor (TC-NER)-driven genome-instability in the pathogenesis of T cell acute lymphoblastic leukemia.  |  R, P., et al. 2024. Clin Immunol. 263: 110204. PMID: 38582251
7. Actin-nucleation promoting factor N-WASP influences alpha-synuclein condensates and pathology.  |  Jackson, J., et al. 2024. Cell Death Dis. 15: 304. PMID: 38693139
8. Wiskott-Aldrich syndrome protein, a novel effector for the GTPase CDC42Hs, is implicated in actin polymerization.  |  Symons, M., et al. 1996. Cell. 84: 723-34. PMID: 8625410
9. Defects in Wiskott-Aldrich syndrome blood cells.  |  Remold-O'Donnell, E., et al. 1996. Blood. 87: 2621-31. PMID: 8639877
10. Direct interaction of the Wiskott-Aldrich syndrome protein with the GTPase Cdc42.  |  Kolluri, R., et al. 1996. Proc Natl Acad Sci U S A. 93: 5615-8. PMID: 8643625
11. Studies of the expression of the Wiskott-Aldrich syndrome protein.  |  Stewart, DM., et al. 1996. J Clin Invest. 97: 2627-34. PMID: 8647957
12. Wiskott-Aldrich syndrome: no strict genotype-phenotype correlations but clustering of missense mutations in the amino-terminal part of the WASP gene product.  |  Schindelhauer, D., et al. 1996. Hum Genet. 98: 68-76. PMID: 8682510

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-WASP Antibody (B-9) has 72 citations in a variety of scientific publications.

Primary antibodies like Anti-WASP Antibody (B-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

WASP Antibody (B-9) Specifications

Alternate Product Name: Wiskott-Aldrich Syndrome Protein

Product Clone Name: B-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-250

Epitope Target: WASP

Epitope Species: human

Product Reactivity/Detection: WASP and N-WASP

Species Reactivity/Detection: mouse, rat, human, mink

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 72 citations in various scientific publications

Available Conjugates/Forms for WASP Antibody (B-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin