WAP Antibody (A-9): sc-398341

WAP Antibody (A-9) is a mouse monoclonal IgM antibody that detects whey acidic protein (WAP) in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). WAP is a hormonally-regulated, acidic, cysteine-rich protein predominantly found in rodent milk, where WAP plays a crucial role in protease activity regulation, contributing to developing neonate protection. WAP is synthesized in mammary epithelial cells and WAP expression significantly increases during pregnancy, driven by lactogenic hormones such as glucocorticoids, prolactin, and insulin. WAP contains two distinct cysteine-rich domains essential for protease inhibitor function, which maintains milk protein integrity and ensures proper offspring nutrition. The WAP gene comprises four exons and three introns, with middle two exons encoding cysteine-rich regions, while a 19-amino-acid N-terminal signal peptide facilitates milk secretion. Although WAP expression occurs primarily in mammary tissue, low WAP mRNA levels can be detected in non-mammary tissues, such as tongue, pancreas, and pituitary gland, suggesting potential roles beyond lactation. WAP secretion in milk remains consistent throughout lactation and is specific to several species, including mouse, rat, rabbit, camel, and porcine, highlighting evolutionary significance in mammalian reproduction and development. Anti-WAP antibody (A-9) serves as an invaluable tool for researchers studying milk protein dynamics and neonatal health implications.

The preferred WAP antibody is WAP Antibody (C-10): sc-374648.