VWA5B2 CRISPR/Cas9 KO Plasmid (h): sc-406578
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VWA5B2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the VWA5B2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VWA5B2 CRISPR/Cas9 KO Plasmid (h) | sc-406578 | 20 µg | $397.00 |
Overview
VWA5B2 encodes a protein containing a von Willebrand factor A (VWA)-like domain, a modular motif often involved in protein–protein interactions that support extracellular matrix organization, adhesion-related signaling, and tissue architecture. Although VWA5B2 is less extensively characterized than many VWA-domain proteins, its predicted domain composition suggests roles in anchoring or scaffolding complexes that influence cellular attachment, motility, and mechanotransduction. Altered regulation of adhesion and matrix-associated processes is frequently linked to dysregulated growth control and invasive phenotypes, making VWA5B2 a useful target for investigating pathways that couple cell–matrix interactions to gene expression and cytoskeletal dynamics. Genetic perturbation of VWA5B2 can therefore help define its contribution to context-dependent stress responses and network rewiring observed in disease-relevant cell states.
VWA5B2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the VWA5B2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the VWA5B2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the VWA5B2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish VWA5B2 protein expression.
This CRISPR knockout system enables efficient generation of VWA5B2-deficient cell models for investigation of VWA5B2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting VWA5B2 exon(s) critical for VWA5B2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple VWA5B2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by VWA5B2 CRISPR/Cas9 KO Plasmid (h) and VWA5B2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the VWA5B2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by VWA5B2 HDR Plasmid (h) and VWA5B2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by VWA5B2 homology arms to support homology-directed repair at defined VWA5B2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.