Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
VRL-1 Double Nickase Plasmid (h): sc-401648-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VRL-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- VRL-1 Double Nickase Plasmid (h) and VRL-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TRPV2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: VRL-1 Antibody (B-9): sc-514848
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VRL-1 Double Nickase Plasmid (h) | sc-401648-NIC | 20 µg | $410.00 | |||
VRL-1 Double Nickase Plasmid (h2) | sc-401648-NIC-2 | 20 µg | $410.00 |
Human TRPV2 encodes the vanilloid receptor-like channel VRL-1, a nonselective, Ca2+-permeable TRP ion channel that integrates mechanical cues, heat, and lipid signaling to shape membrane excitability and intracellular calcium dynamics. VRL-1 activity influences calcium-dependent pathways linked to cytoskeletal remodeling, vesicle trafficking, and downstream MAPK and PI3K-associated signaling that coordinate cell migration, differentiation, and stress responses. The channel is prominently studied in immune and neural cell contexts, where stimulus-evoked Ca2+ influx can modulate inflammatory programs and sensory signaling. Altered TRPV2 expression or channel function has been associated with disease-relevant phenotypes in cancer biology, cardiomyocyte stress responses, and neuroinflammatory processes, supporting its use as a mechanistic target in pathway-focused research.
VRL-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TRPV2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TRPV2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TRPV2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TRPV2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.