VPS51 CRISPR/Cas9 KO Plasmid (h): sc-410709
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VPS51 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the VPS51 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VPS51 CRISPR/Cas9 KO Plasmid (h) | sc-410709 | 20 µg | $397.00 |
Overview
VPS51 encodes a core subunit of the Golgi-associated retrograde protein (GARP) tethering complex that mediates endosome-to-trans-Golgi network retrograde trafficking. By promoting vesicle capture and SNARE-dependent fusion at the TGN, VPS51 helps maintain Golgi organization and supports proper recycling of sorting receptors and cargo proteins. Disruption of GARP function perturbs endolysosomal homeostasis, protein sorting, and glycosylation-dependent maturation pathways, with downstream effects on cellular stress responses. Altered retrograde trafficking is relevant to models of neurobiology, immune signaling, and proliferative phenotypes where membrane transport integrity influences receptor availability and signaling outputs.
VPS51 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the VPS51 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the VPS51 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the VPS51 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish VPS51 protein expression.
This CRISPR knockout system enables efficient generation of VPS51-deficient cell models for investigation of VPS51 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting VPS51 exon(s) critical for VPS51 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple VPS51 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by VPS51 CRISPR/Cas9 KO Plasmid (h) and VPS51 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the VPS51 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by VPS51 HDR Plasmid (h) and VPS51 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by VPS51 homology arms to support homology-directed repair at defined VPS51 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.