VpreB3 CRISPR/Cas9 KO Plasmid (m): sc-423685
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VpreB3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the VpreB3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VpreB3 CRISPR/Cas9 KO Plasmid (m) | sc-423685 | 20 µg | $397.00 |
Overview
Vpreb3 encodes VpreB3, a surrogate light chain–related protein that participates in immunoglobulin assembly and quality control during B cell development. In developing B lineage cells, VpreB3 is linked to endoplasmic reticulum proteostasis and the handling of immunoglobulin light chain components, influencing pre-B cell receptor maturation and downstream signaling competence. Altered regulation of this pathway can impact B cell differentiation states, antibody repertoire formation, and cellular stress responses tied to ER-associated processing. These features make Vpreb3 a useful target for dissecting mechanisms of B cell maturation and immunoglobulin biosynthesis in mouse models.
VpreB3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Vpreb3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Vpreb3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Vpreb3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish VpreB3 protein expression.
This CRISPR knockout system enables efficient generation of Vpreb3-deficient cell models for investigation of VpreB3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Vpreb3 exon(s) critical for VpreB3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Vpreb3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by VpreB3 CRISPR/Cas9 KO Plasmid (m) and VpreB3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Vpreb3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by VpreB3 HDR Plasmid (m) and VpreB3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Vpreb3 homology arms to support homology-directed repair at defined Vpreb3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.