Vitamin D Receptor/VDR Double Nickase Plasmid (h): sc-400171-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Vitamin D Receptor/VDR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Vitamin D Receptor/VDR Double Nickase Plasmid (h) and Vitamin D Receptor/VDR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting VDR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Vitamin D Receptor/VDR Antibody (D-6): sc-13133
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Vitamin D Receptor/VDR Double Nickase Plasmid (h) | sc-400171-NIC | 20 µg | $410.00 | |||
Vitamin D Receptor/VDR Double Nickase Plasmid (h2) | sc-400171-NIC-2 | 20 µg | $410.00 |
VDR encodes the vitamin D receptor, a ligand-activated nuclear receptor that heterodimerizes with RXR and binds vitamin D response elements to regulate transcriptional programs controlling calcium/phosphate homeostasis, epithelial differentiation, and immune modulation. Upon 1,25-dihydroxyvitamin D3 binding, VDR coordinates coactivator/corepressor exchange and integrates with chromatin remodeling to tune gene expression across metabolic and inflammatory pathways. VDR signaling intersects with MAPK, NF-κB, and Wnt/β-catenin networks, linking environmental and hormonal cues to cell-cycle control and stress responses. Altered VDR expression or function has been associated with bone and mineral disorders, autoimmune phenotypes, and tumor biology, motivating mechanistic studies in relevant human cell models.
Vitamin D Receptor/VDR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the VDR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within VDR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt VDR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of VDR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.